Plants from metalliferous soils often exhibit combined tolerance to different heavy metals. Such tolerance could rely either on the possession of a combination of different metal-specific tolerance mechanisms (‘multiple tolerance’), or on less specific mechanisms that pleiotropically confer tolerance to different metals (‘co-tolerance’). In this study, we examined the co-segregation of tolerances to Cu, Zn, Ni, Co and Cd in crosses between distinctly tolerant ecotypes of Silene vulgaris (Moench) Garcke. The results demonstrate non-pleiotropic genetic control of tolerance to Cu, Zn and Cd. Tolerance to Ni and Co, on the other hand, seems to represent the pleiotropic by-product of the tolerance allele of one particular locus for zinc tolerance.