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Molecular analysis of Gigaspora (Glomales, Gigasporaceae)

Published online by Cambridge University Press:  01 July 1998

BERTA BAGO
Affiliation:
Centre de Recherche en Biologie Forestière, Pavillon C-E-Marchand, Université Laval, Québec G1K 7P4, Canada Present address: Eastern Regional Research Center, ARS/USDA, 600 East Mermaid Lane, Wyndmoor, PA 19038, USA. E-mail: [email protected]
STEPHEN P. BENTIVENGA
Affiliation:
Department of Biology and Microbiology, University of Wisconsin Oshkosh, 800 Algoma Bvd., Oshkosh, WI 54901–8640, USA
VIRGINIE BRENAC
Affiliation:
International Institute of Biotechnology, Department of Biosciences, University of Kent, Canterbury, Kent CT2 7YW, UK
JOHN C. DODD
Affiliation:
International Institute of Biotechnology, Department of Biosciences, University of Kent, Canterbury, Kent CT2 7YW, UK
YVES PICHÉ
Affiliation:
Centre de Recherche en Biologie Forestière, Pavillon C-E-Marchand, Université Laval, Québec G1K 7P4, Canada
LUC SIMON
Affiliation:
Recherches en Sciences de la Vie et de la Santé, Pavillon C-E-Marchand, Université Laval, Québec G1K 7P4, Canada
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Abstract

This work presents a cooperative effort to integrate new molecular (isozyme and SSU analyses) characters into the morphological taxonomy of the genus Gigaspora (Glomales). Previous analyses of published Gigaspora SSU sequences indicated the presence of a few polymorphic nucleotides in the region delimited by primers NS71-SSU 1492′. In our study, the SSU of 24 isolates of arbuscular mycorrhizal (AM) fungi from the Gigasporaceae were amplified and the NS71-SSU 1492′ region was directly sequenced. The corresponding sequences of four more isolates of AM fungi from Gigasporaceae, already published, were also included in our analyses. Three Gigaspora groups were identified on the basis of a 6 nucleotide-long ‘molecular signature’: Gigaspora rosea group (G. rosea+G. albida), Gigaspora margarita group (G. margarita+G. decipiens) and Gigaspora gigantea, which constituted a group by itself. The isozyme profiles (malate dehydrogenase, MDH) of 12 of these 28 isolates, and seven other isolates not sequenced, were compared. The results obtained further supported the grouping of isolates provided by the SSU analysis. Both SSU and MDH analysis indicated that two out of the 35 isolates had been misidentified, which was confirmed when their morphology was reassessed. The use of the Gigaspora intrageneric molecular signature as a quick, unambiguous and objective method to recognize Gigaspora isolates under any (field or laboratory) experimental conditions is suggested.

Type
Research Article
Copyright
© Trustees of New Phytologist 1998

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