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N-methyl-d-aspartate receptor independent changes in expression of polysialic acid-neural cell adhesion molecule despite blockade of homosynaptic long-term potentiation and heterosynaptic long-term depression in the awake freely behaving rat dentate gyrus

Published online by Cambridge University Press:  13 August 2009

Jose J. Rodríguez ,
Glenn M. Dallérac ,
Masashi Tabuchi ,
Heather A. Davies ,
Frances M. Colyer ,
Michael G. Stewart  and
Valérie Doyère
Jose J. Rodríguez*
Affiliation:
Faculty of Life Sciences, The University of Manchester, Manchester, UK Institute of Experimental Medicine, ASCR, Prague, Czech Republic
Glenn M. Dallérac
Affiliation:
NAMC, CNRS-UMR8620, Université Paris-Sud, Orsay, France
Masashi Tabuchi
Affiliation:
Faculty of Life Sciences, The University of Manchester, Manchester, UK
Heather A. Davies
Affiliation:
Department of Life Sciences, The Open University, Milton Keynes, UK
Frances M. Colyer
Affiliation:
Department of Life Sciences, The Open University, Milton Keynes, UK
Michael G. Stewart
Affiliation:
Department of Life Sciences, The Open University, Milton Keynes, UK
Valérie Doyère*
Affiliation:
NAMC, CNRS-UMR8620, Université Paris-Sud, Orsay, France
*
Correspondence should be addressed to: V. Doyère, CNRS-UMR8620, NAMC, Université Paris-Sud, Bat. 446, 91405 Orsay, France phone: +33 (0)169 154 987 email: [email protected]; J. J. Rodríguez, Faculty of Life Sciences, The University of Manchester, AV Hill Building, Room 2.002, Oxford Road, Manchester M13 9PT, UK phone: +44 (0)161 275 7324 email: [email protected]
Correspondence should be addressed to: V. Doyère, CNRS-UMR8620, NAMC, Université Paris-Sud, Bat. 446, 91405 Orsay, France phone: +33 (0)169 154 987 email: [email protected]; J. J. Rodríguez, Faculty of Life Sciences, The University of Manchester, AV Hill Building, Room 2.002, Oxford Road, Manchester M13 9PT, UK phone: +44 (0)161 275 7324 email: [email protected]
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Abstract

Investigations examining the role of polysialic acid (PSA) on the neural cell adhesion molecule (NCAM) in synaptic plasticity have yielded inconsistent data. Here, we addressed this issue by determining whether homosynaptic long-term potentiation (LTP) and heterosynaptic long-term depression (LTD) induce changes in the distribution of PSA-NCAM in the dentate gyrus (DG) of rats in vivo. In addition, we also examined whether the observed modifications were initiated via the activation of N-methyl-d-aspartate (NMDA) receptors. Immunocytochemical analysis showed an increase in PSA-NCAM positive cells both at 2 and 24 h following high-frequency stimulation of either medial or lateral perforant paths, leading to homosynaptic LTP and heterosynaptic LTD, respectively, in the medial molecular layer of the DG. Analysis of sub-cellular distribution of PSA-NCAM by electron microscopy showed decreased PSA dendritic labelling in LTD rats and a sub-cellular relocation towards the spines in LTP rats. Importantly, these modifications were found to be independent of the activation of NMDA receptors. Our findings suggest that strong activation of the granule cells up-regulates PSA-NCAM synthesis which then incorporates into activated synapses, representing NMDA-independent plastic processes that act synergistically on LTP/LTD mechanisms without participating in their expression.

Keywords

Adhesion moleculeshippocampussynaptic plasticity
Type
Research Article
Information
Neuron Glia Biology , Volume 4 , Special Issue 3: Cell Adhesion and Extracellular Matrix Molecules in Synaptic Plasticity , August 2008 , pp. 169 - 178
Copyright
Copyright © Cambridge University Press 2009

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