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Ibotenic acid in Amanita muscaria spores and caps

Published online by Cambridge University Press:  23 September 2004

FREDRIK C STØRMER ,
KAREL JANAK  and
GRY E B KOLLER
FREDRIK C STØRMER
Affiliation:
Department of Environmental Medicine, Norwegian Institute of Public Health, P. Nydalen, 0403 Oslo, Norway.
KAREL JANAK
Affiliation:
Department of Environmental Medicine, Norwegian Institute of Public Health, P. Nydalen, 0403 Oslo, Norway.
GRY E B KOLLER
Affiliation:
Division of Molecular Cell Biology, Department of Biology, University of Oslo, P.O. 1045 Blindern, 0316 Oslo, Norway.
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Abstract

Ibotenic acid (α-amino-3-hydroxy-5-isoxazole acetic acid) was separated from spores and caps from Amanita muscaria by reversed phase high performance liquid chromatography and identified by flow injection analysis with mass spectrometric detection. The keto and enol tautomers of ibotenic acid were separated and their ratio of 96:4 in favour of the enol form was determined. On average the ibotenic acid content was 0.0054±0.0010% of the spores and 0.017±0.010% in fresh caps. Muscimol, the decarboxylated product from ibotenic acid, was neither detected in spores nor in caps. 50 nanomol of ibotenic acid, muscimol or extracts from spores or caps did not inhibit the growth of Bacillus subtilis.

Keywords

Amanita muscariabasidiosporesibotenic acidmuscimolneurotoxintautomerism
Type
Original Article
Information
Mycologist , Volume 18 , Issue 3 , August 2004 , pp. 114 - 117
Copyright
© 2004 Cambridge University Press

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