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Visualisation of ectomycorrhizal rhizomorph structure using laser scanning confocal microscopy

Published online by Cambridge University Press:  10 July 2002

Peter F. SCHWEIGER ,
Hervé ROUHIER  and
Bengt SÖDERSTRÖM
Peter F. SCHWEIGER
Affiliation:
Department of Microbial Ecology, Ecology Building, Lund University, S-223 62 Lund, Sweden. Present address: Department of Chemical Physiology of Plants, Vienna University, Althanstraße 14, A-1090 Vienna, Austria.
Hervé ROUHIER
Affiliation:
Department of Microbial Ecology, Ecology Building, Lund University, S-223 62 Lund, Sweden.
Bengt SÖDERSTRÖM
Affiliation:
Department of Microbial Ecology, Ecology Building, Lund University, S-223 62 Lund, Sweden.
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Abstract

A method for the observation of the three-dimensional structure of intact ectomycorrhizal rhizomorphs is described. The method is based on a combination of clearing the material with KOH followed by staining with congo red and subsequent imaging under a laser scanning confocal microscope (LSCM). The images obtained are of a much higher three-dimensional resolution than those obtained previously by use of conventional light microscopical techniques. The structure of highly differentiated and undifferentiated rhizomorphs is described. Applications of the method are briefly discussed.

Type
Research Article
Information
Mycological Research , Volume 106 , Issue 3 , March 2002 , pp. 349 - 354
Copyright
© The British Mycological Society 2002

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