The vegetative life-cycle of Cymadothea trifolii (anamorph Polythrincium trifolii), causing sooty blotch of clover, is described using chemically as well as cryofixed and freeze-substituted samples. The pathogen enters the leaf through stomata and proliferates intercellularly. Nutrients are assumedly obtained via an interaction apparatus produced within the pathogen's hyphae, opposite to which the host cell is triggered to invaginate its plasmalemma. Rare attempts of ‘self-parasitism’ were also seen. Entering the conidial stage, stromata are laid down under the lower epidermis. The dying tissue above may explain the necrotic spots observed on infected leaflets. Foot cells in the conidial stromata produce thick-walled conidiophores, which grow sympodially. New conidiophores may grow into empty shells of old ones. Conidia are detached after pores between them and conidiophores have become plugged by organelles resembling Woronin bodies. Conidia are usually two-celled and their walls contain chitin and β-1,3-glucans as indicated by labelling with gold-conjugated wheat germ agglutinin and anti-β-1,3-glucan antibodies. Both conidiophores and conidia contain a structure which we regard as a new organelle with as yet unknown function.