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Use of molecular methods to estimate the size and distribution of mycelial individuals of the ectomycorrhizal basidiomycete Pisolithus tinctorius

Published online by Cambridge University Press:  01 March 1998

I. C. ANDERSON ,
S. M. CHAMBERS  and
J. W. G. CAIRNEY
I. C. ANDERSON
Affiliation:
Department of Biological Sciences, University of Western Sydney (Nepean), PO Box 10, Kingswood, NSW 2747, Australia
S. M. CHAMBERS
Affiliation:
Department of Biological Sciences, University of Western Sydney (Nepean), PO Box 10, Kingswood, NSW 2747, Australia
J. W. G. CAIRNEY
Affiliation:
Department of Biological Sciences, University of Western Sydney (Nepean), PO Box 10, Kingswood, NSW 2747, Australia
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Abstract

A field study was conducted to determine the size and spatial distribution of mycelial individuals of Pisolithus tinctorius at a site in NSW, Australia. Following collection and mapping of carpophores and isolation of the fungi into axenic culture, genomic DNA was extracted and combined data from RAPD and microsatellite analyses used to identify and map mycelial individuals. Thirty-three genetically distinct individuals were recognized at the field site and, while one large individual (at least 30 m diam.) was identified, most individuals appeared relatively small (<3 m diam.). The size and distribution of individuals is discussed in relation to growth of P. tinctorius mycelia through soil.

Type
Research Article
Information
Mycological Research , Volume 102 , Issue 3 , March 1998 , pp. 295 - 300
Copyright
The British Mycological Society 1998

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