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Unencapsidated double-stranded RNA associated with membrane vesicles in isolates of Alternaria solani

Published online by Cambridge University Press:  01 May 1997

I. ZABALGOGEAZCOA ,
D. PETRUNAK ,
B. J. CHRIST  and
F. E. GILDOW
I. ZABALGOGEAZCOA
Affiliation:
Department of Plant Pathology, The Pennsylvania State University, University Park, PA 16802, U.S.A. Instituto de Recursos Naturales y Agrobiología, CSIC, Apartado 257, 37071 Salamanca, Spain
D. PETRUNAK
Affiliation:
Department of Plant Pathology, The Pennsylvania State University, University Park, PA 16802, U.S.A.
B. J. CHRIST
Affiliation:
Department of Plant Pathology, The Pennsylvania State University, University Park, PA 16802, U.S.A.
F. E. GILDOW
Affiliation:
Department of Plant Pathology, The Pennsylvania State University, University Park, PA 16802, U.S.A.
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Abstract

Double-stranded RNA (dsRNA) elements of 8·3 and 5·5 kbp were detected in three of five geographically distinct isolates of Alternaria solani. Variation with respect to the number of dsRNA segments was detected among isolates of the fungus. No virus-like particles were purified from the mycelium, or observed in ultra-thin sections of hyphae. Utilizing differential centrifugation or density gradient centrifugation for subcellular fractionation, dsRNA was found associated specifically with fractions containing 80–100 nm spherical membrane vesicles. Similar vesicles were observed in cytoplasm of hyphae by electron microscopy. Treatment of dsRNA-containing fractions with ribonuclease A did not degrade the dsRNA, suggesting that it is located inside the vesicles. There was no correlation between the radial growth of the isolates in laboratory media and the presence or absence or dsRNA.

Type
Research Article
Information
Mycological Research , Volume 101 , Issue 5 , May 1997 , pp. 604 - 608
Copyright
The British Mycological Society 1997

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