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Tyrosinase activity and isoform composition in separate tissues during development of Agaricus bisporus fruit bodies

Published online by Cambridge University Press:  01 April 1999

JEROEN VAN LEEUWEN  and
HARRY J. WICHERS
JEROEN VAN LEEUWEN
Affiliation:
Agrotechnological Research Institute (ATO-DLO), Bornsesteeg 59, 6708 PD Wageningen, The Netherlands
HARRY J. WICHERS
Affiliation:
Agrotechnological Research Institute (ATO-DLO), Bornsesteeg 59, 6708 PD Wageningen, The Netherlands
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Abstract

During growth of Agaricus bisporus fruit bodies the amount of active tyrosinase increased. The amount of active tyrosinase can be related to the degree of browning, as opposed to the fully activated tyrosinase level. Isoelectric focusing revealed that active and latent tyrosinase isoforms having different isoelectric points could be distinguished. The isoform composition was dependent on the tissue and developmental stage. In the later developmental stages new active isoforms appeared.

Type
Research Article
Information
Mycological Research , Volume 103 , Issue 4 , April 1999 , pp. 413 - 418
Copyright
The British Mycological Society 1999

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