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Transformation of the fungal forest pathogen Dothistroma pini to hygromycin resistance

Published online by Cambridge University Press:  01 October 1997

R. E. BRADSHAW ,
A. BIDLAKE ,
N. FORESTER  and
D. B. SCOTT
R. E. BRADSHAW
Affiliation:
Molecular Genetics Unit, Department of Microbiology and Genetics, Massey University, Palmerston North, New Zealand
A. BIDLAKE
Affiliation:
Molecular Genetics Unit, Department of Microbiology and Genetics, Massey University, Palmerston North, New Zealand
N. FORESTER
Affiliation:
Molecular Genetics Unit, Department of Microbiology and Genetics, Massey University, Palmerston North, New Zealand
D. B. SCOTT
Affiliation:
Molecular Genetics Unit, Department of Microbiology and Genetics, Massey University, Palmerston North, New Zealand
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Abstract

The forest pathogen Dothistroma pini, which produces the polyketide toxin, dothistromin, was transformed with the pAN7-1 plasmid containing the Escherichia coli hygromycin B phosphotransferase gene (hph). The frequency of hygromycin resistant transformants was 9–48 μg−1 DNA. Southern blot analysis indicated that a single copy of the vector DNA had integrated into the genome in at least six of the eight transformants tested and that the site of integration was different in each case. All transformants analysed were mitotically stable through several subcultures without selective pressure.

Type
Research Article
Information
Mycological Research , Volume 101 , Issue 10 , October 1997 , pp. 1247 - 1250
Copyright
The British Mycological Society 1997

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