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Temperature regulation of extracellular proteases in ectomycorrhizal fungi (Hebeloma spp.) grown in axenic culture

Published online by Cambridge University Press:  01 June 1999

M. TIBBETT
Affiliation:
School of Conservation Sciences, Bournemouth University, Talbot Campus, Poole, Dorset BH12 5BB, U.K.
F. E. SANDERS
Affiliation:
School of Biology, University of Leeds, Leeds LS2 9JT, U.K.
J. W. G. CAIRNEY
Affiliation:
Mycorrhiza Research Group, School of Science, University of Western Sydney, Nepean, PO Box 10, Kingswood, NSW 2747, Australia
J. R. LEAKE
Affiliation:
Department of Animal and Plant Sciences, University of Sheffield, Sheffield, S10 2TN, U.K.
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Abstract

Strains of Hebeloma representative of different climatic zones were grown in axenic culture at either 2°C and 22° or 6° and 22°. Culture filtrates were assayed for proteolytic activity using FITC labelled BSA as a substrate. Assays were run between 0–37°. Growth at low temperature induced greater proteolytic activity (g−1D.W. mycelium). Many of the strains produced protease(s) which retained significant activity at temperatures as low as 0°, and a thermal optimum between 0–6° with a second optimum at higher temperature. The results are discussed in relation the nutrient acquisition potential of ectomycorrhizal fungi at low temperature and the contribution such cold active proteases might make to the soil enzyme pool.

Type
Research Article
Copyright
© The British Mycological Society 1999

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