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Techniques for selective isolation, semiquantification and rapid virulence testing of Catenaria anguillulae

Published online by Cambridge University Press:  01 June 1998

K. P. SINGH
Affiliation:
Department of Mycology and Plant Pathology, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi – – 221 005, India
P. BANDYOPADHYAY
Affiliation:
Department of Mycology and Plant Pathology, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi – – 221 005, India
R. A. STEPHEN
Affiliation:
Department of Mycology and Plant Pathology, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi – – 221 005, India
S. S. VAISH
Affiliation:
Department of Mycology and Plant Pathology, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi – – 221 005, India
T. MAKESH KUMAR
Affiliation:
Department of Mycology and Plant Pathology, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi – – 221 005, India
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Abstract

Techniques for selective isolation, semiquantification and rapid virulence testing are described for Catenaria anguillulae, an important endoparasitic fungus of nematodes. Selective isolation was achieved by using second-stage juveniles of Anguina tritici as bait in soil aliquots for 48 h. Semiquantitative estimation of the population of the fungus was made by calculating the infection percentage of inoculated juveniles. Rapid virulence testing was done by using second-stage juveniles of Heterodera cajani, Meloidogyne incognita and A. tritici.

Type
Research Article
Copyright
© The British Mycological Society 1998

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