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A resource-saving method for isolation of Fusarium and other fungi from individual soil particles

Published online by Cambridge University Press:  01 December 1999

HELLE HESTBJERG ,
SUSANNE ELMHOLT ,
ULF THRANE  and
ULLA B. JENSEN
HELLE HESTBJERG
Affiliation:
Danish Institute of Agricultural Sciences, Department of Crop Physiology and Soil Science, Research Centre Foulum, P.O. Box 50, DK-8830 Tjele, Denmark
SUSANNE ELMHOLT
Affiliation:
Danish Institute of Agricultural Sciences, Department of Crop Physiology and Soil Science, Research Centre Foulum, P.O. Box 50, DK-8830 Tjele, Denmark
ULF THRANE
Affiliation:
Department of Biotechnology, Technical University of Denmark, DK-2800 Lyngby, Denmark
ULLA B. JENSEN
Affiliation:
Danish Institute of Agricultural Sciences, Department of Crop Physiology and Soil Science, Research Centre Foulum, P.O. Box 50, DK-8830 Tjele, Denmark
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Abstract

Plating of individual soil particles onto an agar medium, poured into Petri dishes, is a method of studying fungal distribution between microhabitats in soil. The substitution of Petri dishes with well plates reduced both the required amount of agar medium and labour. A microshovel prepared from an injection needle facilitated the handling of individual, small size, soil particles (0·25–0·50 mm). The described method was evaluated in a study of the distribution of Fusarium within two wheat field soils from Ås, Norway, and from Østermarie, Denmark. Four types of organic particles were distinguished: light coloured root pieces, dark coloured root pieces, straw pieces, and miscellaneous organic pieces. The dominating species were F. culmorum, F. oxysporum and F. avenaceum. Aspects on the value of the method with regard to fungal substrate preference are discussed.

Type
Research Article
Information
Mycological Research , Volume 103 , Issue 12 , December 1999 , pp. 1545 - 1548
Copyright
© The British Mycological Society 1999

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