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Proteomic analysis of asexual development of Phytophthora palmivora

Published online by Cambridge University Press:  20 May 2003

Samantha J. SHEPHERD ,
Pieter VAN WEST  and
Neil A. R. GOW
Samantha J. SHEPHERD
Affiliation:
Department of Molecular and Cell Biology, Institute of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen AB25 2ZD, Scotland, UK. E-mail: [email protected] Present address: Department of Biology (Area 9), University of York, PO Box 373, York, YO10 5YW, UK.
Pieter VAN WEST
Affiliation:
Department of Molecular and Cell Biology, Institute of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen AB25 2ZD, Scotland, UK. E-mail: [email protected]
Neil A. R. GOW
Affiliation:
Department of Molecular and Cell Biology, Institute of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen AB25 2ZD, Scotland, UK. E-mail: [email protected]
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Abstract

Two-dimensional gel electrophoresis was used to analyse stage-specific proteins from Phytophthora palmivora, a pathogen of cocoa and other economically important tropical crops. Approximately 1% of proteins appeared to be specific for each of the mycelial, sporangial, zoospore, cyst and germinated cyst stages of the life-cycle. Three proteins excised from protein gels of P. palmivora were identified as isoforms of actin by database searches to public libraries of Phytophthora infestans. The protein profiles of parallel samples of P. palmivora and P. infestans demonstrated that 30% of proteins precisely co-migrated suggesting that proteomics may be used to examine changes in the specific stages in the life cycles of Phytophthora spp.

Type
Research Article
Information
Mycological Research , Volume 107 , Issue 4 , April 2003 , pp. 395 - 400
Copyright
© The British Mycological Society 2003

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