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Phylogenetic relationships among some cercosporoid anamorphs of Mycosphaerella based on rDNA sequence analysis

Published online by Cambridge University Press:  01 November 1999

ELWIN L. STEWART ,
ZHAOWEI LIU ,
PEDRO W. CROUS  and
LES J. SZABO
ELWIN L. STEWART
Affiliation:
Department of Plant Pathology, The Pennsylvania State University, Buckhout Laboratory, University Park, PA, 16802, U.S.A.
ZHAOWEI LIU
Affiliation:
Department of Plant Pathology, The Pennsylvania State University, Buckhout Laboratory, University Park, PA, 16802, U.S.A.
PEDRO W. CROUS
Affiliation:
Department of Plant Pathology, University of Stellenbosch, P. Bag X1, Matieland 7602, South Africa
LES J. SZABO
Affiliation:
USDA ARS Cereal Disease Laboratory, 1551 Lindig Street, St Paul, MN 55108, U.S.A.
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Abstract

Partial rDNA sequences were obtained from 26 isolates representing species of Cercospora, Passalora, Paracercospora, Pseudocercospora, Ramulispora, Pseudocercosporella and Mycocentrospora. The combined internal transcribed spacers (ITS) including the 5·8S rRNA gene and 5′ end of the 25S gene (primer pairs F63/R635) on rDNA were amplified using PCR and sequenced directly. The ITS regions including the 5·8S varied in length from 502 to 595 bp. The F63/R635 region varied from 508 to 519 bp among isolates sequenced. Reconstructed phylogenies inferred from both regions had highly similar topologies for the taxa examined. Phylogenetic analysis of the sequences resulted in four well-supported clades corresponding to Cercospora, Paracercospora/Pseudocercospora, Passalora and Ramulispora, with bootstrap values greater than 92% for each clade. Based on the results of the analysis, a new combination for Pseudocercosporella aestiva is proposed in Ramulispora, and Paracercospora is reduced to synonymy with Pseudocercospora.

Type
Research Article
Information
Mycological Research , Volume 103 , Issue 11 , November 1999 , pp. 1491 - 1499
Copyright
The British Mycological Society 1999

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