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Phylogenetic analysis of the phytopathogenic fungus Fusarium solani based on the rDNA-ITS region

Published online by Cambridge University Press:  21 November 2000

H. SUGA ,
T. HASEGAWA ,
H. MITSUI ,
K. KAGEYAMA  and
M. HYAKUMACHI
H. SUGA
Affiliation:
Molecular Genetics Research Center, Gifu University, Gifu 501-1112, Japan
T. HASEGAWA
Affiliation:
Laboratory of Plant Pathology, Faculty of Agriculture, Gifu University, Gifu 501-1112, Japan
H. MITSUI
Affiliation:
Institute of Genetic Ecology, Tohoku University. Katahira, Aobaku, Sendai 980-8577, Japan. E-mail: [email protected]
K. KAGEYAMA
Affiliation:
Laboratory of Plant Pathology, Faculty of Agriculture, Gifu University, Gifu 501-1112, Japan
M. HYAKUMACHI
Affiliation:
Laboratory of Plant Pathology, Faculty of Agriculture, Gifu University, Gifu 501-1112, Japan
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Abstract

The nucleotide sequence of the internal transcribed spacer region of the ribosomal RNA gene (rDNA-ITS) was obtained for strains belonging to 10 special forms (f. spp.) of the phytopathogenic fungus Fusarium solani (teleomorph Haematonectria haematococca). Phylogenetic analysis of nucleotide sequences of the rDNA-ITS region showed that most clusters were composed of strains belonging to the same special form. F. solani f. sp. eumartii and f. sp. phaseoli were excluded from the cluster composed of all other special forms. In order to use the molecular data to identify each special form, several restriction enzymes specific for each special form were selected from the mapping data. The digestion pattern of the rDNA-ITS region with these restriction enzymes was able to distinguish F. solani f. spp. phaseoli, cucurbitae race 1, batatas, piperis, eumartii, and xanthoxyli. Although f. spp. mori, robiniae and pisi were not differentiated by restriction analysis, they were separated in the phylogenetic tree.

Type
Research Article
Information
Mycological Research , Volume 104 , Issue 10 , October 2000 , pp. 1175 - 1183
Copyright
© The British Mycological Society 2000

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