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Nucleotide composition in protein-coding and non-coding DNA in the zygomycete Phycomyces blakesleeanus

Published online by Cambridge University Press:  13 August 2004

Luis M. CORROCHANO  and
Javier RUIZ-ALBERT
Luis M. CORROCHANO
Affiliation:
Departamento de Genética, Facultad de Biología, Universidad de Sevilla, Apartado 1095, ES-41080 Sevilla, Spain. E-mail: [email protected]
Javier RUIZ-ALBERT
Affiliation:
Departamento de Genética, Facultad de Biología, Universidad de Sevilla, Apartado 1095, ES-41080 Sevilla, Spain. E-mail: [email protected] Current address: Departamento de Biología Celular, Genética y Fisiología, Universidad de Málaga, Campus de Teatinos, ES-29071 Málaga, Spain.
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Abstract

The zygomycete Phycomyces blakesleeanus has a 30 Mb genome with a 35% content of guanine and cytosine (GC). We determined the GC content in Phycomyces genes and fragments of genes available in public databases, the frequency of nucleotides in each codon position, and the codon usage. We observed a difference of 18% between the GC content of protein-coding and non-coding DNA. This large difference allowed the visualization of protein-coding DNA by plotting the GC content along a segment of Phycomyces DNA. We have identified a high GC DNA segment linked to the pyrG genes of the zygomycete genera Phycomyces, Mucor, and Blakeslea that corresponds to the 3′ end of the gene responsible for the protein kinase C.

Type
Research Article
Information
Mycological Research , Volume 108 , Issue 8 , August 2004 , pp. 858 - 863
Copyright
The British Mycological Society 2004

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