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Molecular analysis of intraspecific variation between building and ‘wild’ isolates of Serpula lacrymans and their relatedness to S. himantioides

Published online by Cambridge University Press:  17 May 2001

Nia A. WHITE ,
Prabhjyot K. DEHAL ,
James M. DUNCAN ,
Naomi A. WILLIAMS ,
Jill S. GARTLAND ,
John W. PALFREYMAN  and
David E. L. COOKE
Nia A. WHITE
Affiliation:
Division of Molecular and Life Sciences, School of Science and Engineering, University of Abertay Dundee, Bell Street, Dundee DD1 1HG, UK
Prabhjyot K. DEHAL
Affiliation:
Division of Molecular and Life Sciences, School of Science and Engineering, University of Abertay Dundee, Bell Street, Dundee DD1 1HG, UK Present address: Medical Oncology, Paterson Institute of Cancer Research, Christie Hospital, Wilmslow Road, Manchester 20 9BX, UK
James M. DUNCAN
Affiliation:
Fungal and Bacterial Plant Pathology Department, Scottish Crop Research Institute, Invergowrie, Dundee DD2 5DA, UK
Naomi A. WILLIAMS
Affiliation:
Fungal and Bacterial Plant Pathology Department, Scottish Crop Research Institute, Invergowrie, Dundee DD2 5DA, UK
Jill S. GARTLAND
Affiliation:
Division of Molecular and Life Sciences, School of Science and Engineering, University of Abertay Dundee, Bell Street, Dundee DD1 1HG, UK
John W. PALFREYMAN
Affiliation:
Division of Molecular and Life Sciences, School of Science and Engineering, University of Abertay Dundee, Bell Street, Dundee DD1 1HG, UK
David E. L. COOKE
Affiliation:
Fungal and Bacterial Plant Pathology Department, Scottish Crop Research Institute, Invergowrie, Dundee DD2 5DA, UK
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Abstract

Relationships between Serpula lacrymans and S. himantioides isolates of ‘wild’ Himalayan woodland and building (European and Australian) origin were examined using RAPD–PCR and ITS rDNA sequence comparison. GelCompar analysis of the genetic fingerprints of eighteen isolates using four RAPD-primer combinations clearly distinguished S. lacrymans and S. himantioides. Little intraspecific variation was observed in S. lacrymans (including isolates of Himalayan woodland origin), relative to S. himantioides. Despite previously reported differences in growth rates and decay capabilities of ‘wild’ Himalayan woodland S. lacrymans isolates and those from buildings, RAPD fingerprints and ITS sequences indicated they were genetically similar. Interspecific ITS sequence polymorphisms were consistent with other studies and clearly demonstrated S. himantioides and S. lacrymans were distinct taxa. Evolutionary questions regarding the origins of S. lacrymans are discussed.

Type
Research Article
Information
Mycological Research , Volume 105 , Issue 4 , April 2001 , pp. 447 - 452
Copyright
© The British Mycological Society 2001

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