Chaetopsina hongkongensis sp. nov. from decaying palm rachids, and Kionochaeta australiensis sp. nov. from decaying wood submerged in a freshwater stream, are described and illustrated. Both species produced setose conidiophores and phialidic conidiogenous cells. The former is distinct in having polyblastic conidiogenous cells with multiple denticulate loci, and is compared with C. polyblastia. In Kionochaeta australiensis conidiophores have accessory setiform lateral branches, and it is compared with K. keniensis, K. pughii, and K. ramifera. The delimitation of these two genera is discussed with additional information provided from an SEM study of conidiogenous characters in C. hongkongensis.