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Ectomycorrhizal mycobionts of Pisonia grandis on coral cays in the Capricorn-Bunker group, Great Barrier Reef, Australia

Published online by Cambridge University Press:  22 September 2005

Susan M. CHAMBERS ,
Catherine J. HITCHCOCK  and
John W. G. CAIRNEY
Susan M. CHAMBERS
Affiliation:
Centre for Horticulture and Plant Sciences, Parramatta Campus, University of Western Sydney, Locked Bag 1797, Penrith South DC, NSW 1797, Australia. E-mail: [email protected]
Catherine J. HITCHCOCK
Affiliation:
Centre for Horticulture and Plant Sciences, Parramatta Campus, University of Western Sydney, Locked Bag 1797, Penrith South DC, NSW 1797, Australia. E-mail: [email protected]
John W. G. CAIRNEY
Affiliation:
Centre for Horticulture and Plant Sciences, Parramatta Campus, University of Western Sydney, Locked Bag 1797, Penrith South DC, NSW 1797, Australia. E-mail: [email protected]
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Abstract

The diversity of ectomycorrhizal mycobionts of Pisonia grandis (Nyctaginaceae) from coral cays in the Capricorn-Bunker group, Great Barrier Reef, Australia, was examined. Only two ectomycorrhiza morphotypes (brown and black) were identified in soil from seven cays and DNA from both morphotypes was subjected to ITS-RFLP and sequence analysis. The brown morphotype was present in soil from all cays but the black morphotype was only observed in soil from three cays. ITS-RFLP analysis showed that the brown and black morphotypes were formed by different fungal taxa, with the RFLP pattern for the black morphotype being consistent with that of the culture previously obtained from black ectomycorrhizal roots on Heron Island. Comparison with the GenBank database revealed that closest matches to both morphotypes were sequences for various Thelephoraceae (Basidiomycota), but the brown and black morphotypes had only 80% sequence similarity to each other. Neighbour-joining analysis of these sequences with sequences for other Thelephoraceae grouped the brown and black morphotypes in a well-supported clade with several Tomentella species, suggesting that both belong to this genus. The data are discussed in relation to ectomycorrhizal fungal diversity and the coral cay habitat.

Type
Research Article
Information
Mycological Research , Volume 109 , Issue 10 , October 2005 , pp. 1105 - 1111
Copyright
© The British Mycological Society 2005

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