Mycelial preparations of Erynia neoaphidis were tested under different drying and storage conditions. For unformulated mycelial discs and formulated alginate granules, sporulation was best maintained if drying at 20–22 °C was performed at 60 or 90% r.h. rather than an ambient humidity of 30%. Storage experiments were performed on preparations dried at 90% r.h. for 72 h. Estimates for a 50% decline in sporulation with storage time (ST50) were computed if there were statistically significant fits using exponential or non-parametric linear regression. For mycelial discs, values of ST50 were between 36–39 d when maintained at 10° with silica gel or 20% r.h., and 14–26 d when kept at 10° and 35% r.h. or with silica gel and 15 or 20°. For alginate granules, ST50 values of 6–9 d were obtained at 10° and 20–55% r.h. A 5- to 9-fold increase in sporulation was produced with granules containing 2% w/v sucrose and 5% w/v starch compared to granules without additives. Elevated humidities are recommended for drying E. neoaphidis mycelia and viability of dried preparations is best maintained if they are stored at 10° compared with 15 or 20°.