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DNA fingerprinting and vegetative compatibility analysis indicate multiple origins for Verticillium dahliae race 2 tomato isolates from Ontario, Canada

Published online by Cambridge University Press:  01 September 1998

KATHERINE F. DOBINSON ,
NII A. PATTERSON ,
GERARD J. WHITE  and
SANDRA GRANT
KATHERINE F. DOBINSON
Affiliation:
Agriculture and Agri-Food Canada, Pest Management Research Centre, London, ON, Canada N5V 4T3 Department of Plant Sciences, The University of Western Ontario, London, ON, Canada N6A 5B7 Department of Microbiology and Immunology, The University of Western Ontario, London, ON, Canada N6A 5C1
NII A. PATTERSON
Affiliation:
Agriculture and Agri-Food Canada, Pest Management Research Centre, London, ON, Canada N5V 4T3 Current address: Plant Biotechnology Centre, Department of Biological Sciences, University of Calgary, Calgary, AB, Canada T2N 1N4.
GERARD J. WHITE
Affiliation:
Department of Plant Sciences, The University of Western Ontario, London, ON, Canada N6A 5B7
SANDRA GRANT
Affiliation:
Agriculture and Agri-Food Canada, Pest Management Research Centre, London, ON, Canada N5V 4T3
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Abstract

RFLP, DNA fingerprinting and VCG methods were used to characterize fourteen Verticillium dahliae isolates collected from southwestern Ontario, Canada. The isolates were typed as not pathogenic to tomato (NP), race 1 (avirulent on cvs carrying the Ve resistance gene) or race 2 (virulent on Ve cvs). On the basis of RFLPs, RAPDs, and DNA fingerprints detected by hybridization to a dispersed, repetitive genomic DNA probe, the isolates were classified into five DNA types. Type I included two NP isolates. Type II included four race 2, and three NP isolates. Types III and V were represented by single race 2 and race 1 isolates, respectively. Type IV included one race 2, and two race 1 isolates. Vegetative compatibility was determined for selected NP, race 1, and race 2 isolates of each race type/DNA type combination. Isolates of the same DNA type were compatible, as were type II and III isolates (VCG 4B), and type IV (VCG 2A) and V isolates (VCG 2B). These data show a level of genetic diversity not previously identified in the V. dahliae tomato pathogen population, and suggest multiple origins for the Ontario race 2 pathotype.

Type
Research Article
Information
Mycological Research , Volume 102 , Issue 9 , September 1998 , pp. 1089 - 1095
Copyright
The British Mycological Society 1998

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