Hostname: page-component-6bf8c574d5-mcfzb Total loading time: 0 Render date: 2025-03-09T04:28:53.326Z Has data issue: false hasContentIssue false
  • English
  • Français

Detection of Tricholoma matsutake by specific ITS primers

Published online by Cambridge University Press:  17 January 2001

Kensuke KIKUCHI ,
Norihisa MATSUSHITA ,
Alexis GUERIN-LAGUETTE ,
Akira OHTA  and
Kazuo SUZUKI
Kensuke KIKUCHI
Affiliation:
Laboratory of Forest Botany, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan
Norihisa MATSUSHITA
Affiliation:
Arboricultural Research Institute, University Forests, The University of Tokyo, 457 Kanou, Minamiizu-cho, Kamo-gun, Shizuoka 415-0304, Japan
Alexis GUERIN-LAGUETTE
Affiliation:
Laboratory of Forest Botany, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan
Akira OHTA
Affiliation:
Shiga Forest Research Centre, Kitasakura, Yasu-cho, Shiga 520-2321, Japan
Kazuo SUZUKI
Affiliation:
Laboratory of Forest Botany, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan
Get access

Abstract

Sequences of the internal transcribed spacer (ITS) region of the nuclear ribosomal DNA of ectomycorrhizal fungi were analysed and a specific PCR primer pair for Tricholoma matsutake was designed. Using the primer pair, part of the ITS region of T. matsutake (400 bp) was amplified, but no amplified fragment was detected for other ectomycorrhizal fungi. PCR was performed on DNA extracted from mycorrhizas of T. matsutake and Shiro soil (extramatrical mycelium of T. matsutake and soil complexes) and the 400 bp fragments were also specifically amplified. This indicates that presence of T. matsutake at any of its life stages can be easily confirmed by PCR typing.

Type
Research Article
Information
Mycological Research , Volume 104 , Issue 12 , December 2000 , pp. 1427 - 1430
Copyright
© The British Mycological Society 2000

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)