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Colonisation and molecular diversity of arbuscular mycorrhizal fungi in the aquatic plants Littorella uniflora and Lobelia dortmanna in southern Sweden

Published online by Cambridge University Press:  14 June 2004

Kit Bjerregaard NIELSEN
Affiliation:
Institute of Biology, University of Southern Denmark, Campusvej 55, DK-5230 Odense M, Denmark.
Rasmus KJØLLER
Affiliation:
Department of Mycology, Botanical Institute, Øster Farimagsgade 2D, DK-1353 Copenhagen K, Denmark.
Pål Axel OLSSON
Affiliation:
Department of Microbial Ecology, Ecology Building, Lund University, SE-223 62 Lund, Sweden. E-mail: [email protected]
Peter F. SCHWEIGER
Affiliation:
Department of Chemistry and Physiology of Plants, University of Vienna, Althanstr. 14, A-1090 Vienna, Austria.
Frede Ø. ANDERSEN
Affiliation:
Institute of Biology, University of Southern Denmark, Campusvej 55, DK-5230 Odense M, Denmark.
Søren ROSENDAHL
Affiliation:
Department of Mycology, Botanical Institute, Øster Farimagsgade 2D, DK-1353 Copenhagen K, Denmark.
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Abstract

The colonisation intensity and composition of the mycorrhizal community in the aquatic plants Lobelia dortmanna and Littorella uniflora were studied. The mycorrhizal fungi were characterised by fungal specific nested PCR and sequencing using the 5′-end of the LSU rDNA as target. For this, primers for the clade of Acaulospora, the clade including Glomus mosseae and G. intraradices and the clade containing G. etunicatum and G. claroideum were used.

The nested PCR products were screened for different sequence types using single stranded conformation polymorphism (SSCP) and representatives for each type were sequenced. A phylogenetic analysis of the sequences showed two phylotypes of Acaulospora, one phylotype within the clade of G. etunicatum/G. claroideum and five within the G. mosseae/G. intraradices clade. The colonisation intensity was comparable to that seen in typical grassland vegetation. The neutral lipid fatty acid 16[ratio ]1ω5 was seen to be indicative of mycorrhizal colonisation with concentrations up to 35 nmol mg−1 root DW, which indicates that the fungi are active.

Type
Research Article
Copyright
© The British Mycological Society 2004

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