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Agrobacterium and PEG-mediated transformation of the phytopathogen Venturia inaequalis

Published online by Cambridge University Press:  12 August 2003

Anna M. FITZGERALD ,
Agnieszka M. MUDGE ,
Andrew P. GLEAVE  and
Kim M. PLUMMER
Anna M. FITZGERALD
Affiliation:
School of Biological Sciences, University of Auckland, Private Bag 92019, Auckland, New Zealand. E-mail: [email protected]
Agnieszka M. MUDGE
Affiliation:
Gene Technologies, The Horticulture and Food Research Institute of New Zealand Ltd, Private Bag 92169, Auckland, New Zealand. Current address: CSIRO Plant Industry, Long Pocket Laboratories, 120 Meiers Rd, Indooroopilly, Qld, 4068, Australia.
Andrew P. GLEAVE
Affiliation:
Gene Technologies, The Horticulture and Food Research Institute of New Zealand Ltd, Private Bag 92169, Auckland, New Zealand.
Kim M. PLUMMER
Affiliation:
School of Biological Sciences, University of Auckland, Private Bag 92019, Auckland, New Zealand. E-mail: [email protected]
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Abstract

We report the development of two new transformation systems, polyethylene glycol (PEG)-mediated transformation of protoplasts and Agrobacterium tumefaciens-mediated transformation of mycelium, for the filamentous ascomycete Venturia inaequalis. New binary vectors have been created for the latter. Although transformation was initially achieved using a PEG-mediated method, this was superseded by the A. tumefaciens-mediated approach. The advantages of the latter include: ease of the protocol, no requirement for protoplasts; higher transformation efficiency; and single-site integration. A comparison between the two transformation methods is presented.

Type
Research Article
Information
Mycological Research , Volume 107 , Issue 7 , July 2003 , pp. 803 - 810
Copyright
© The British Mycological Society 2003

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