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Transformation of the edible fungi, Pleurotus ostreatus and Volvariella volvacea

Published online by Cambridge University Press:  01 July 1998

JIAN HUA JIA ,
JOHN A. BUSWELL  and
JOHN F. PEBERDY
JIAN HUA JIA
Affiliation:
Department of Biology, Chinese University of Hong Kong, Shatin, New Territories, Hong Kong
JOHN A. BUSWELL
Affiliation:
Department of Biology, Chinese University of Hong Kong, Shatin, New Territories, Hong Kong
JOHN F. PEBERDY
Affiliation:
Department of Biology, Chinese University of Hong Kong, Shatin, New Territories, Hong Kong Permanent address: Department of Life Science, University of Nottingham, Nottingham NG7 2RD, U.K.
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Abstract

A transformation system is described for the edible mushrooms Pleurotus ostreatus and Volvariella volvacea. The system developed is based on a positive selection strategy using the trp3iar gene from Coprinus cinereus, which confers resistance to the antimetabolite 5-fluoroindole. Transformation frequencies were low in both species. Southern blot analysis confirmed the integration of transforming DNA into the genome of transformants and indicated the presence of tandemly duplicated copies of the plasmid in some of these transformants. The system has potential for introducing beneficial traits such as enhanced substrate bioconversion and faster sporophore development.

Type
Research Article
Information
Mycological Research , Volume 102 , Issue 7 , July 1998 , pp. 876 - 880
Copyright
The British Mycological Society 1998

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