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Production of a monoclonal antibody specific to Mycena galopus mycelium

Published online by Cambridge University Press:  01 September 1997

PAULA HITCHCOCK ,
TIMOTHY R. G. GRAY  and
JULIET C. FRANKLAND
PAULA HITCHCOCK
Affiliation:
Present address: Cymbus Biotechnology Ltd, Unit J, Eagle Close, Chandler's Ford, Hampshire SO53 4NF,U.K. Department of Biology, University of Essex, Wivenhoe Park, Colchester, CO4 3SQ, U.K. Institute of Terrestrial Ecology, Merlewood Research Station, Grange-over-Sands, Cumbria LA11 6JU, U.K.
TIMOTHY R. G. GRAY
Affiliation:
Department of Biology, University of Essex, Wivenhoe Park, Colchester, CO4 3SQ, U.K.
JULIET C. FRANKLAND
Affiliation:
Institute of Terrestrial Ecology, Merlewood Research Station, Grange-over-Sands, Cumbria LA11 6JU, U.K.
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Abstract

Monoclonal antibodies were raised against the mycelium of a litter-decomposing basidiomycete, Mycena galopus, for use in an autecological study of the fungus in forest litter. Two clones (3E12 and 6D8), produced using particulate and whole-cell extracts of the fungus respectively, cross-reacted with several species including other Mycena species, non-Mycena basidiomycetes, zygomycetes and anamorphs or mitosporic fungi. Of these clones, 3E12 appeared to be directed against an unidentified β-glucan and 6D8 against chitin. A third clone (6E1), using as immunogen latex collected in the field from stipes of the basidiomes, was specific to M. galopus mycelium in ELISA and immunofluorescence tests and was probably directed against an unidentified polysaccharide.

Type
Research Article
Information
Mycological Research , Volume 101 , Issue 9 , September 1997 , pp. 1051 - 1059
Copyright
The British Mycological Society 1997

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