One hundred and thirty four dikaryotic isolates of Ganoderma, morphologically determined as G. adspersum, G. annulare, G. applanatum, G. brownii, G. lobatoideum, G. lobatum, G. lucidum, G. oerstedii, G. resinaceum, and G. tornatum, were examined by horizontal PAGE for eight enzymatic activities. A total of 94 bands were analysed. The objective was to evaluate the relatedness among the specific taxa by analysing numerically the isoenzymic patterns. To discern fungal individuals somatic incompatibility tests were used. Within each enzyme system several allozyme phenotypes could be observed. The enzymic systems analysed failed to yield diagnostic bands for each morphologically defined group, except the AKP system for G. resinaceum. Some bands were shared by all the isolates studied. On the other hand, diagnostic bands at the species complex level were encountered.