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Detection of multiple rRNA-ITS regions in isolates of Ascochyta

Published online by Cambridge University Press:  01 June 1998

JAMSHID FATEHI  and
PAUL BRIDGE
JAMSHID FATEHI
Affiliation:
International Mycological Institute, Bakeham Lane, Egham, Surrey TW20 9TY, U.K.
PAUL BRIDGE
Affiliation:
International Mycological Institute, Bakeham Lane, Egham, Surrey TW20 9TY, U.K.
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Abstract

Internally transcribed spacer (ITS) regions of the rRNA gene cluster in isolates of Ascochyta spp. were amplified and digested with restriction endonucleases. Addition of fragment sizes from conventional gel electrophoresis gave anomalous sizes which suggested the presence of more than one PCR product. Separation in HA-Yellow containing agarose gel allowed the detection of fragments of the same size but with different sequences. Isolates of Ascochyta spp. were shown to possess multiple PCR-amplification products of the same sizes, but with significant sequence differences.

Type
Research Article
Information
Mycological Research , Volume 102 , Issue 6 , June 1998 , pp. 762 - 766
Copyright
© The British Mycological Society 1998

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