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Biochemical and molecular characterization of South African strains of Phanerochaete chrysosporium

Published online by Cambridge University Press:  01 January 1998

THEODORUS H. DE KOKER ,
JIONG ZHAO ,
DAN CULLEN  and
BERNARD J. H. JANSE
THEODORUS H. DE KOKER
Affiliation:
Department of Microbiology, University of Stellenbosch, Stellenbosch 7600, South Africa
JIONG ZHAO
Affiliation:
Department of Microbiology, University of Stellenbosch, Stellenbosch 7600, South Africa
DAN CULLEN
Affiliation:
Institute for Microbial and Biochemical Technology, U.S. Department of Agriculture, Forest Products Laboratory, Madison, Wisconsin, U.S.A.
BERNARD J. H. JANSE
Affiliation:
Department of Microbiology, University of Stellenbosch, Stellenbosch 7600, South Africa
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Abstract

Fifty-five strains of Phanerochaete chrysosporium were isolated in South Africa, and screened for indicators of ligninolytic activity: lignin peroxidase (LiP), manganese peroxidase (MnP) and glyoxal oxidase (GLOX). MnP-production as a function of time was followed in all strains. Nine strains were selected for quantification of MnP, LiP and GLOX activities. Statistically significant variation in MnP and GLOX activities existed among the different strains. Under low nitrogen, LiP activity of selected strains showed no significant variation, whereas strain PP25 had significantly increased LiP levels under high nitrogen conditions. Probing genomic DNA with the genes encoding lignin peroxidase (lipD and lipI1), manganese peroxidase (mnp2), and glyoxal oxidase (glox) showed significant genetic diversity with lignin peroxidase and manganese peroxidase probes, but not with the glyoxal oxidase probe.

Type
Research Article
Information
Mycological Research , Volume 102 , Issue 1 , January 1998 , pp. 88 - 92
Copyright
© The British Mycological Society 1998

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