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Using Hydrothermal Method to Prepare Reduced Graphene-Hemin Electrochemical Biosensor for Tyrosine Detection

Published online by Cambridge University Press:  12 April 2013

Junhua Wei  and
Jenny Qiu
Junhua Wei
Affiliation:
Department of Mechanical Engineering, Texas Tech University, 2500 Broadway, Lubbock, TX, 79409-1021, U.S.A.
Jenny Qiu
Affiliation:
Department of Mechanical Engineering, Texas Tech University, 2500 Broadway, Lubbock, TX, 79409-1021, U.S.A.
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Abstract

Hemin immobilized reduced graphene(HGN) has been investigated to be an outstanding enzymatic catalysis in detection important molecular recently. In this work, two "clean" methods to prepare HGN through π-π stack were charactered by UV-vis spectra, TEM images, and δ-potential. The enzymatic catalysis of both materials was compared by catalytic hydrogen peroxide to oxidize pyrogallol. The colorimetric result shows HGN attached before reduction has stronger catalytic ability than the one after reduction. The optimized HGN was then used as an electrochemical biosensor to determine L-tyrosine levels. The cyclic voltammetry (CV) tests were carried out for the bare glass carbon electrode (GCE), and the optimized hemin-reduced graphene electrode (HGN1/GCE). The HGN1/GCE based biosensor exhibits a Tyrosine detection linear range from 5×10-7 M to 4×10-5 M with a detection limitation of 7.5×10-8 M at signal noise ratio (S/N) of 3. In comparison with other biosensor, electrochemical biosensors are easy-fabricated, easy-controlled, and cost-effective. Compared with other materials, the hemin-reduced graphene based biosensors demonstrate higher stability, a broader detection linear range, and better detection sensitivity. The study of oxidation scheme reveals that reduced graphene enhanced the electron transfer between electrode and hemin. Meanwhile, the hemin groups effectively electrocatalyzed the oxidation of tyrosine. This study contributes to a widespread clinical application of nanomaterial based biosensor devices with a broader detection linear range, improved stability, enhanced sensitivity, and reduced costs.

Keywords

biomaterialCcatalytic
Type
Articles
Information
MRS Online Proceedings Library (OPL) , Volume 1505: Symposium W – Carbon Nanomaterials , 2013 , mrsf12-1505-w10-34
Copyright
Copyright © Materials Research Society 2013

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References

REFERENCES

Groot, M. T. D., Merkx, M., Wonders, A. H., and Koper, M. T. M., J. OF THE AMERICAN CHEMICAL SOCIETY. 127, 7579 (2005).CrossRefGoogle Scholar
Duong, B., Arechabaleta, R., and Tao, N. J., J. of Electroanalytical Chemistry. 447, 63 (1998).CrossRefGoogle Scholar
Brusova, Z., and Magner, E., Bioelectrochemistry. 75, 63 (2009).CrossRefGoogle Scholar
Mimica, D., Zagal, J. H., and Bedioui, F., J. OF ELECTROANALYTICAL CHEMISTRY. 497, 106 (2001).CrossRefGoogle Scholar
Ye, J. S., Wen, Y., Zhang, W. D., Cui, H. F., Gan, L. M., Xu, G. Q., and Sheu, F. S., J. OF ELECTROANALYTICAL CHEMISTRY. 562, 241 (2004).CrossRefGoogle Scholar
Zheng, N., Zeng, Y., Osbome, P.G., Li, Y., Chang, W., and Wang, Z., J. Appl. Electrochem. 32, 129 (2002).CrossRefGoogle Scholar
Zhang, F., Zheng, B., Zhang, J. L., Huang, X. L., Liu, H., Guo, S. W., and Zhang, J. Y., J. OF PHYSICAL CHEMISTRY C. 114, 8469 (2010).CrossRefGoogle Scholar
Valentini, F., Cristofanelli, L., Carbone, M., and Palleschi, G., ELECTROCHIMICA ACTA. 63, 37 (2012).CrossRefGoogle Scholar
Travascio, P., Li, Y., and Sen, D., Chemistry Biology. 5, 505 (1998).CrossRefGoogle Scholar
Wang, S., Zhang, Y., and Abidi, N., LANGMUIR. 25, 11078 (2009).CrossRefGoogle Scholar
Qiu, J., and Wang, S., Journal of Applied Polymer Science. 119, 3670 (2011).CrossRefGoogle Scholar
Raee, M. A., Raee, J., Wang, Z., Song, H., Yu, Z., and Koratkar, N., ACS Nano. 3, 3884 (2009).Google Scholar
Mattevi, C., Eda, G., Agnoli, S., and Miller, S., Adv. Funct. Mater. 19, 2577 (2009).CrossRefGoogle Scholar
Eda, G. and Chhowalla, M., Adv. Mater. 22, 2392 (2010).CrossRefGoogle Scholar
Rao, C. N. R., Sood, A. K., Subrahmanyam, K. S., Govindaraj, A., and Govindaraj, A., ANGEWANDTE CHEMIE-INTERNATIONAL EDITION. 48, 7752 (2009).CrossRefGoogle Scholar
Xu, Y., Bai, H., Lu, G., Lu, , Li, C., and Shi, G., J. OF THE AMERICAN CHEMICAL SOCIETY. 130, 5856 (2008).CrossRefGoogle Scholar
Geng, J. X., H. and Jung, T., J. OF PHYSICAL CHEMISTRY C. 114, 8227 (2010).CrossRefGoogle Scholar
Ji, C., Lu, Z., Hua, B., and Shi, G., J. OF ELECTROANALYTICAL CHEMISTRY. 657, 34 (2011).Google Scholar
Guo, Y., Deng, L., Li, J., Guo, S., Wang, E., and Dong, S., ACS Nano. 5, 1282 (2011).CrossRefGoogle Scholar
Wei, J., Qiu, J., Li, L., Ren, L., Zhang, X., Chaudhuri, J. and Wang, S., Nanotechnology. 23, 335707 (2012).CrossRefGoogle Scholar
Wang, S., Tambraparni, M., Qiu, J., Tipton, J. and Dean, D., Macromolecules. 42, 5251 (2009).CrossRefGoogle Scholar
Dan, L., Muller, M. B., Gilje, S., Kaner, R. B. and Wallace, G. G., Nature nanotechnology. 3, 101 (2008).Google Scholar
Ma, Q., Ai, S., Yin, H., Chen, Q. and Tang, T., Electrochimica Acta. 55, 6687 (2010).CrossRefGoogle Scholar
Silver, J. and Lukas, B., Inorganica Chim. Acta. 78, 205 (1987).Google Scholar
Tanga, X., Liua, Y., Houb, H., and Youa, T., Talanta 80 2182 (2010).CrossRefGoogle Scholar