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Use of Flexible Materials with a Novel Pressure Driven Equibiaxial Cell Stretching Device for Mechanical Stimulation of Single Mammalian Cells

Published online by Cambridge University Press:  15 February 2011

James D. Kubicek ,
Stephanie Brelsford  and
Philip R. LeDuc
James D. Kubicek
Affiliation:
Department of Mechanical Engineering Carnegie Mellon University Pittsburgh, Pennsylvania 15213, U.S.A.
Stephanie Brelsford
Affiliation:
Department of Mechanical Engineering Carnegie Mellon University Pittsburgh, Pennsylvania 15213, U.S.A.
Philip R. LeDuc
Affiliation:
Department of Mechanical Engineering Carnegie Mellon University Pittsburgh, Pennsylvania 15213, U.S.A.
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Abstract

Mechanical stimulation of single cells has been shown to affect cellular behavior from the molecular scale to ultimate cell fate including apoptosis and proliferation. In this, the ability to control the spatiotemporal application of force on cells through their extracellular matrix connections is critical to understand the cellular response of mechanotransduction. Here, we develop and utilize a novel pressure-driven equibiaxial cell stretching device (PECS) combined with an elastomeric material to control specifically the mechanical stimulation on single cells. Cells were cultured on silicone membranes coated with molecular matrices and then a uniform pressure was introduced to the opposite surface of the membrane to stretch single cells equibiaxially. This allowed us to apply mechanical deformation to investigate the complex nature of cell shape and structure. These results will enhance our knowledge of cellular and molecular function as well as provide insights into fields including biomechanics, tissue engineering, and drug discovery.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 773: Symposium N – Biomicroelectromechanical Systems (BioMEMS) , 2003 , N5.6
Copyright
Copyright © Materials Research Society 2003

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