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Uncoiling of DNA by Double Chained Cationic Surfactants

Published online by Cambridge University Press:  10 February 2011

H. H. Paradies  and
S. F. Clancy
H. H. Paradies
Affiliation:
Biotechnology & Physical Chemistry, Märkische Fachhochschule, Iserlohn, D- 58644, Germany, [email protected]
S. F. Clancy
Affiliation:
Witco Corporation, Safety, Health, & Environmental Affairs, One American Lane, Greenwich, CT 06831–2559, USA, [email protected]
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Abstract

Distearyldimethylammonium (DSDMA) X (X− = OH, H2PO4) interact with double stranded T4 DNA (166 kilobase pairs) below and above the CMC (1.5×10−6). Below the CMC of either DSDMA X where the cationic double-chained surfactants are in the monomeric state, T4 DNA and DSDMA+form a compact complex where all surfactant molecules are bound. Close to the CMC, particularly for DSDMA OH but also in the presence of , T4 DNA exhibits a condensed (globule) conformation, though some DNA molecules are in the more extended DNA conformation. Above the CMC of DSDMA X a plateau is reached (up to 3.0 × 10−6 M surfactant) revealing a penetration of DSDMA molecules into the DNA globules resulting in a loosening of the tightened state of DNA. The various stages of the transition from the condensed coil to the extended state of T4 DNA with changing DSDMA X concentrations was monitored by static and inelastic light scattering experiments which were supplemented by small-angle X-ray scattering measurements.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 463: Symposia EE – Statistical Mechanics in Physics and Biology , 1996 , 49
Copyright
Copyright © Materials Research Society 1997

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References

REFERENCES

[1] Cohen, S.S., Adv. Polyamine Res., 1, 1–10, (1978);Google Scholar
Gosule, L. C. & Schellman, , Nature, 259, 33, (1976);Google Scholar
Gosule, L.C. & Schellman, J.A., J. Mol. Biol., 121, 311, (1979);Google Scholar
Wilson, R.W. & Bloomfield, V.A., Biochemistry, 18, Biochemistry, 18, 2192, (1979)Google Scholar
[2] Riemer, S.C. & Bloomfield, V.A., Biopoymers, 17, 785, (1978)Google Scholar
[3] Manning, G.S., Q. Rev. Biophys., 11, 179, (1978)Google Scholar
[4] Manning, G.S., J. Phys. Chem., 85, 870, (1981)Google Scholar
[5] Paradies, H.H., Thies, M., Clancy, S.F., this symposium “Statistical Mechanics in Physics & Biology, MRS, eds. Wirtz, D., Halsey, T.C., van Zanten, J., (1996)Google Scholar
[6] Clancy, S.F., Steiger, P.H., Tanner, D.A., Thies, M., Paradies, H.H., J. Phys. Chem., 98, 11143, (1994);Google Scholar
Thies, M., Clancy, S.F., Paradies, H.H., J. Phys. Chem., 100, 9881, (1996);Google Scholar
Paradies, H.H., J. Phys. Chem., 90, 5956, (1986)Google Scholar
[7] Felgner, P.L. et al., Proc. Natl. Acad. Sci. (USA), 84, 7413, (1987)Google Scholar
[8] Paradies, H.H., US-Pat. # 4,074, 850 (1989);Google Scholar
Paradies, H.H. & Schroer, U., Pharm. Ind., 12, 1387, (1988);Google Scholar
Clancy, S.F., Tanner, D.A., Thies, M., Paradies, H.H., Extended Abstracts, Div. of Environ. Chem., S.F.,CA, 203rd Natl. Meet. of the ACS, Vol 32, No 1, 907–909, (1992)Google Scholar
[9] Me'nikov, S.M., Sergeyer, V.G., Yoshikawa, K., J. Am. ehem. Soc, B7, 2401, (1995)Google Scholar
[10] Thomas, C.A. & Abelson, J., in “Procedure in Nucleic Acid Research”, eds. Cantoni, G.C., Davies, P.R., Vol. 553, Harper, N.Y. (1966)Google Scholar
[11] Marmur, J., Methods Enzymol., 6, 726, (1963)Google Scholar
[12] Gerhard, B. & Warren, R.A.J., Biochemistry, 21, 5458, (1982)Google Scholar
[13] Post, C.B. & Zimm, B.H., Biopolymers, 21, 2139, (1982);Google Scholar
Benbasat, A., Biochemistry, 23, 3609, (1984)Google Scholar
[14] Paradies, H.H., Colloids & Surfaces, 74, 57, (1994)Google Scholar
[15] Allison, S.A., Herr, J.C., Teller, D.C., Biopolymers, 19, 1475, (1980).Google Scholar