Hostname: page-component-78c5997874-t5tsf Total loading time: 0 Render date: 2024-11-05T12:15:36.628Z Has data issue: false hasContentIssue false

Uncoiling of DNA by Double Chained Cationic Surfactants

Published online by Cambridge University Press:  10 February 2011

H. H. Paradies
Affiliation:
Biotechnology & Physical Chemistry, Märkische Fachhochschule, Iserlohn, D- 58644, Germany, [email protected]
S. F. Clancy
Affiliation:
Witco Corporation, Safety, Health, & Environmental Affairs, One American Lane, Greenwich, CT 06831–2559, USA, [email protected]
Get access

Abstract

Distearyldimethylammonium (DSDMA) X (X = OH, H2PO4) interact with double stranded T4 DNA (166 kilobase pairs) below and above the CMC (1.5×10−6). Below the CMC of either DSDMA X where the cationic double-chained surfactants are in the monomeric state, T4 DNA and DSDMA+form a compact complex where all surfactant molecules are bound. Close to the CMC, particularly for DSDMA OH but also in the presence of , T4 DNA exhibits a condensed (globule) conformation, though some DNA molecules are in the more extended DNA conformation. Above the CMC of DSDMA X a plateau is reached (up to 3.0 × 10−6 M surfactant) revealing a penetration of DSDMA molecules into the DNA globules resulting in a loosening of the tightened state of DNA. The various stages of the transition from the condensed coil to the extended state of T4 DNA with changing DSDMA X concentrations was monitored by static and inelastic light scattering experiments which were supplemented by small-angle X-ray scattering measurements.

Type
Research Article
Copyright
Copyright © Materials Research Society 1997

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

[1] Cohen, S.S., Adv. Polyamine Res., 1, 110, (1978);Google Scholar
Gosule, L. C. & Schellman, , Nature, 259, 33, (1976);Google Scholar
Gosule, L.C. & Schellman, J.A., J. Mol. Biol., 121, 311, (1979);Google Scholar
Wilson, R.W. & Bloomfield, V.A., Biochemistry, 18, Biochemistry, 18, 2192, (1979)Google Scholar
[2] Riemer, S.C. & Bloomfield, V.A., Biopoymers, 17, 785, (1978)Google Scholar
[3] Manning, G.S., Q. Rev. Biophys., 11, 179, (1978)Google Scholar
[4] Manning, G.S., J. Phys. Chem., 85, 870, (1981)Google Scholar
[5] Paradies, H.H., Thies, M., Clancy, S.F., this symposium “Statistical Mechanics in Physics & Biology, MRS, eds. Wirtz, D., Halsey, T.C., van Zanten, J., (1996)Google Scholar
[6] Clancy, S.F., Steiger, P.H., Tanner, D.A., Thies, M., Paradies, H.H., J. Phys. Chem., 98, 11143, (1994);Google Scholar
Thies, M., Clancy, S.F., Paradies, H.H., J. Phys. Chem., 100, 9881, (1996);Google Scholar
Paradies, H.H., J. Phys. Chem., 90, 5956, (1986)Google Scholar
[7] Felgner, P.L. et al., Proc. Natl. Acad. Sci. (USA), 84, 7413, (1987)Google Scholar
[8] Paradies, H.H., US-Pat. # 4,074, 850 (1989);Google Scholar
Paradies, H.H. & Schroer, U., Pharm. Ind., 12, 1387, (1988);Google Scholar
Clancy, S.F., Tanner, D.A., Thies, M., Paradies, H.H., Extended Abstracts, Div. of Environ. Chem., S.F.,CA, 203rd Natl. Meet. of the ACS, Vol 32, No 1, 907909, (1992)Google Scholar
[9] Me'nikov, S.M., Sergeyer, V.G., Yoshikawa, K., J. Am. ehem. Soc, B7, 2401, (1995)Google Scholar
[10] Thomas, C.A. & Abelson, J., in “Procedure in Nucleic Acid Research”, eds. Cantoni, G.C., Davies, P.R., Vol. 553, Harper, N.Y. (1966)Google Scholar
[11] Marmur, J., Methods Enzymol., 6, 726, (1963)Google Scholar
[12] Gerhard, B. & Warren, R.A.J., Biochemistry, 21, 5458, (1982)Google Scholar
[13] Post, C.B. & Zimm, B.H., Biopolymers, 21, 2139, (1982);Google Scholar
Benbasat, A., Biochemistry, 23, 3609, (1984)Google Scholar
[14] Paradies, H.H., Colloids & Surfaces, 74, 57, (1994)Google Scholar
[15] Allison, S.A., Herr, J.C., Teller, D.C., Biopolymers, 19, 1475, (1980).Google Scholar