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Ultrathin Films of Oriented Bacteriorhodopsin: Nanostructured Films for Investigating the Primary Photoevent in Vision Processes

Published online by Cambridge University Press:  21 March 2011

Rigoberto C. Advincula  and
Mi-kyoung Park
Rigoberto C. Advincula
Affiliation:
Department of Chemistry, University of Alabama at Birmingham, Birmingham, Al 35294-1240, USA
Mi-kyoung Park
Affiliation:
Department of Chemistry, University of Alabama at Birmingham, Birmingham, Al 35294-1240, USA
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Abstract

In this work, a protocol for investigating Bacteriorhodopsin (BR) biomimetic systems as ultrathin films is presented. BR is one of the most well studied proteins important for investigating the primary photo-event in vision processes. The use of macromolecular assembly approaches for deposition onto solid support substrates, e.g. SiOx, gold- or ITO-coated glass (electrode) provide advantages in that surface sensitive measurements can be used to correlate photocurrent generation, photoelectric response, pH change, chromophore behavior, etc. with protein orientation at interfaces. Membrane and protein morphology were correlated to measurements using surface sensitive techniques, such as atomic force microscopy (AFM), ellipsometry, quartz crystal microbalance (QCM), etc. on solid-substrate systems. These studies can lead to applications in optobioelectronic devices (biosensors) including patterning in transducer array configurations, where the film structure is important. Hybrid films are possible with supramolecular assembly approaches, e.g. adsorption of membrane with lipidbilayers. We report our initial results on highly ordered and oriented BR protein arrays of controlled thickness, layer order, and orientation. This was done primarily using the alternate polyelectrolyte deposition (APD) or layer-by-layer (LbL) approach to functionalize substrate surfaces.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 679: Symposium B – Molecular and Biomolecular Electronics , 2001 , B3.2
Copyright
Copyright © Materials Research Society 2001

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References

REFERENCES

1. Ulman, A., An Introduction to Ultrathin Organic Films: From Langmuir Blodgett to Self Assembly, Academic Press: Boston, 1991.Google Scholar
2. (a) Oesterhelt, D., Stoeckenius, W., Nature, New Biol. 149, 233, (1971). (b) W. Stoeckenius, R.A. Bogomolni, Annu. Rev. Biochem., 52, 587 (1982).Google Scholar
3. (a) Birge, R., Annu. Rev. Phys. Chem. 41, 683 (1990) and References cited therein.(b) C. Brauchle, N. Hampp, D. Oesterhelt, Adv. Mater. 3, 420 (1991). (c) D. Oesterhelt, C. Brauchle, N. Hampp, Q. Rev. Biophys. 24, 425 (1991) and References cited therein.Google Scholar
4. (a) Miyasaka, T., Koyama, K. Appl. Opt. 32, 6371 (1993). (b) T. Miyasaka, K. Koyama, I. Otoh, Science 255, 342 (1992).Google Scholar
5. (a) Birge, R.R. Am. Sci. 82, 348 (1994)., (b) M. Wolperdinger, N. Hampp, Biophys. Chem. 56, 189 (1995).Google Scholar
6. Miyasaka, T., Koyama, K. Thin Solid Films 210/211, 146 (1992).Google Scholar
7. Keszthelyi, L., Biochim. Biophys. Acta 598, 429 (1980).Google Scholar
8. Brizzolara, R., BioSystems 35, 137 (1995).Google Scholar
9. Koyama, K., Yamaguchi, N., Miyasaka, T., Science 265, 762 (1994).Google Scholar
10. Koyama, K., Yamaguchi, N., Miyasaka, T., Adv. Mater. 7, 590 (1995).Google Scholar
11. Decher, G., Hong, J., Makromol. Chem. 46, 321 (1992).Google Scholar
12. Decher, G., Science 277, 1232 (1997).Google Scholar
13. Ferreira, F., Rubner, M., Macromolecules 28, 7107 (1995).Google Scholar
14. He, J. A., Samuelson, L., Li, L., Kumar, J., Tripathy, S.K. Langmuir 14, 1674 (1998).Google Scholar
15. Hampp, N., Chem. Rev. 100, 1755 (2000).Google Scholar