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Translocation of N-acetyl Cysteine Capped Fluorescent Quantum Dots in Plant Tissue: Confocal Imaging Studies

Published online by Cambridge University Press:  08 April 2015

Smruti Das ,
Jeremy Tharkur ,
Laurene Tetard  and
Swadeshmukul Santra
Smruti Das
Affiliation:
NanoScience Technology Center, University of Central Florida, 12424 Research Parkway, Suite 400, Orlando, FL 32826.
Jeremy Tharkur
Affiliation:
NanoScience Technology Center, University of Central Florida, 12424 Research Parkway, Suite 400, Orlando, FL 32826. Burnett School of Biomedical Sciences, University of Central Florida, 12424 Research Parkway, Suite 400, Orlando, FL 32826.
Laurene Tetard
Affiliation:
NanoScience Technology Center, University of Central Florida, 12424 Research Parkway, Suite 400, Orlando, FL 32826. Department of Physics, University of Central Florida, 12424 Research Parkway, Suite 400, Orlando, FL 32826.
Swadeshmukul Santra*
Affiliation:
NanoScience Technology Center, University of Central Florida, 12424 Research Parkway, Suite 400, Orlando, FL 32826. Burnett School of Biomedical Sciences, University of Central Florida, 12424 Research Parkway, Suite 400, Orlando, FL 32826. Department of Materials Science and Engineering and University of Central Florida, 12424 Research Parkway, Suite 400, Orlando, FL 32826. Department of Chemistry, University of Central Florida, 12424 Research Parkway, Suite 400, Orlando, FL 32826.
*
*Corresponding Author: [email protected]
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Abstract

Semiconductor fluorescent quantum dots (Qdots) are popularly used as bioimaging taggants in live cell imaging and spectroscopy. In recent years, Qdots taggants are emerging in agricultural applications. Studies are primarily focused on nanotoxicity of ultra-small size water-soluble Qdots in plant systems. Nanotoxicity is correlated with Qdot core composition and surface coating. However, Qdots with certain chemical composition and surface coating may boost plant growth. In this study, we report that N-acetyl cysteine (NAC) capped ∼3.5 nm size ZnS:Mn/ZnS Qdots (NAC-Qdot) are efficiently uptaken by the snow pea (Pisum sativum L., a model plant) vascular system, enhancing the root growth at a dose level of 80 μg/mL. Fluorescence microscopy studies confirmed localization of NAC-Qdots in the intercellular regions. Germination and growth of the snow pea seeds were found to be strongly dependent on Qdot dosage and incubation time with Qdots. Seed germination reached 100% within 48 hours of NAC-Qdot exposure. Based on our preliminary findings, it is suggested that NAC-Qdot can be used as systemic plant nutrient material for boosting the seed germination and plant growth.

Keywords

luminescencenanostructuresemiconducting
Type
Articles
Information
MRS Online Proceedings Library (OPL) , Volume 1754: Symposium OO/PP/QQ/TT – State-of-the-Art Developments in Materials Characterization , 2015 , pp. 43 - 51
Copyright
Copyright © Materials Research Society 2015 

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