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Tissue Engineering of Fibroblast Constructs and Anisotropic Collagen Gels

Published online by Cambridge University Press:  01 February 2011

Sarah Calve ,
Ellen Arruda ,
Robert Dennis ,
Karl Grosh  and
Krystyna Pasyk
Sarah Calve
Affiliation:
Macromolecular Science and Engineering Center Institute of Gerontology University of Michigan Ann Arbor, MI 48109
Ellen Arruda
Affiliation:
Department of Mechanical Engineering
Robert Dennis
Affiliation:
Department of Mechanical Engineering Institute of Gerontology University of Michigan Ann Arbor, MI 48109
Karl Grosh
Affiliation:
Department of Mechanical Engineering
Krystyna Pasyk
Affiliation:
Institute of Gerontology University of Michigan Ann Arbor, MI 48109
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Abstract

The creation of an in vitro functional tendon construct will enable testing of the influence of mechanics and nutrients on the development and remodeling of tendon under known controlled stimuli which is difficult to achieve in vivo. Tendon constructs were engineered in vitrovia stress-mediated self organization of fibroblasts and ECM on a laminin coated elastomer substrate. Varying the laminin density and the amount of fetal bovine serum on the substrate affected the ability of tendon fibroblasts to form a confluent cell layer and the time to layer delamination. Understanding the factors that promote self-assembly of tendon constructs will enable their combination with already developed in vitro muscle constructs.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 724: Symposium N – Biological and Biomimetic Materials Properties to Function , 2002 , N7.9
Copyright
Copyright © Materials Research Society 2002

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References

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