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Tissue Engineering of Fibroblast Constructs and Anisotropic Collagen Gels

Published online by Cambridge University Press:  01 February 2011

Sarah Calve
Affiliation:
Macromolecular Science and Engineering Center Institute of Gerontology University of Michigan Ann Arbor, MI 48109
Ellen Arruda
Affiliation:
Department of Mechanical Engineering
Robert Dennis
Affiliation:
Department of Mechanical Engineering Institute of Gerontology University of Michigan Ann Arbor, MI 48109
Karl Grosh
Affiliation:
Department of Mechanical Engineering
Krystyna Pasyk
Affiliation:
Institute of Gerontology University of Michigan Ann Arbor, MI 48109
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Abstract

The creation of an in vitro functional tendon construct will enable testing of the influence of mechanics and nutrients on the development and remodeling of tendon under known controlled stimuli which is difficult to achieve in vivo. Tendon constructs were engineered in vitrovia stress-mediated self organization of fibroblasts and ECM on a laminin coated elastomer substrate. Varying the laminin density and the amount of fetal bovine serum on the substrate affected the ability of tendon fibroblasts to form a confluent cell layer and the time to layer delamination. Understanding the factors that promote self-assembly of tendon constructs will enable their combination with already developed in vitro muscle constructs.

Type
Research Article
Copyright
Copyright © Materials Research Society 2002

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References

[1] Dennis, R. G., and Kosnik, P. E., “Excitability and Isometric Contractile Properties of Mammalian Skeletal Muscle Constructs Engineered In Vitro ,” In Vitro Cellular Developmental Biology - Animal, vol. 36 pp. 327335, 2000.Google Scholar
[2] Russell, J. E., and Manske, P. R., “Ascorbic Acid Requirement for Optimal Flexor Tendon Repair In Vitro ,” Journal of Orthopaedic Research, vol. 9 pp. 714719, 1991.Google Scholar
[3] Stewart, D. M., “The Role of Tension in Muscle Growth,” in Goss, R. J., (ed.) Regulation of Organ and Tissue Growth 1972, pp. 77100.Google Scholar
[4] Danowski, B. A., “Microtubule Dynamics in Serum-Starved and Serum-Stimulated Swiss 3T3 Mouse Fibroblasts: Implications for the Relationship Between Serum-Induced Contractility and Microtubules,” Cell Motility and the Cytoskeleton, vol. 40 pp. 112, 1998.Google Scholar
[5] Kinoshita, Y. and Kuzuhara, T., “Soft tissue reaction to collagen-immobilized porous polyethylene: subcutaneous implantatation in rats for 20 wk,” Biomaterials, vol. 14, no. 3, pp. 209215, 1992.Google Scholar
[6] Swasdison, S. and Mayne, R., “ In Vitro Attachment of Skeletal Muscle Fibers to a Collagen Gel Duplicates the Structure of the Myotendinous Junction,”; Experimental Cell Research, vol. 193 pp. 227231, 1991.Google Scholar
[7] Doane, K. J., and Birk, D. E., “Fibroblasts Retain Their Tissue Phenotype When Grown in Three-Dimensional Collagen Gels,” Experimental Cell Research, vol. 195 pp. 432442, 1991.Google Scholar
[8] Kardon, G., “Muscle and Tendon Morphogenesis in the Avian Hind Limb,” Development, vol. 125 pp. 40194032, 1998.Google Scholar