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Thin film micro arrays with immobilized DNA for hybridization analysis

Published online by Cambridge University Press:  01 February 2011

F. Fixe ,
A. Faber ,
D. Gonçalves ,
D.M.F. Prazeres ,
R. Cabeça ,
V. Chu ,
G. Ferreira  and
J.P. Conde
F. Fixe
Affiliation:
Center for Biological & Chemical Engineering, Instituto Superior Técnico, Lisbon, Portugal INESC Microsystems and Nanotechnologies, Lisbon, Portugal
A. Faber
Affiliation:
Center for Biological & Chemical Engineering, Instituto Superior Técnico, Lisbon, Portugal
D. Gonçalves
Affiliation:
Center for Biological & Chemical Engineering, Instituto Superior Técnico, Lisbon, Portugal
D.M.F. Prazeres
Affiliation:
Center for Biological & Chemical Engineering, Instituto Superior Técnico, Lisbon, Portugal
R. Cabeça
Affiliation:
INESC Microsystems and Nanotechnologies, Lisbon, Portugal
V. Chu
Affiliation:
INESC Microsystems and Nanotechnologies, Lisbon, Portugal
G. Ferreira
Affiliation:
Faculdade de Engenharia de Recursos Naturais, Universidade do Algarve, Faro, Portugal
J.P. Conde
Affiliation:
Department of Materials Engineering, Instituto Superior Técnico, Lisbon, Portugal
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Abstract

In this work, a procedure to immobilize DNA probes on a microarray patterned on a flexible plastic substrate is developed. The method involves the chemical activation of a thin film surface, the introduction of amine functionality via a silanization step, the coupling of an adequate crosslinker and finally the immobilization of the DNA probe. The response of different thin-film materials and plastic substrates to the immobilization procedure is discussed. The DNA probes immobilized in the patterned pixels were then allowed to hybridize with complementary target DNA labeled with a fluorescent molecule. A prototype array of thin film pixels of SiO2 functionalized by silanization deposited over a polyimide substrate is demonstrated.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 723: Symposia M/O – Molecularly Imprinted Materials – Chemical and Biological Sensors-Materials and Devices , 2002 , O2.3
Copyright
Copyright © Materials Research Society 2002

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