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Synthesis of Liposome Reinforced with Cholesterol and Application to Transmission Electron Microscopy Observation

Published online by Cambridge University Press:  12 March 2013

Marina Kamogawa ,
Takuji Ube ,
Junichi Shimanuki ,
Takashi Harumoto ,
Makoto Yuasa  and
Takashi Ishiguro
Marina Kamogawa
Affiliation:
Department of Materials Science and Technology, Faculty of Industrial Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-8510, Japan
Takuji Ube
Affiliation:
Department of Materials Science and Technology, Faculty of Industrial Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-8510, Japan
Junichi Shimanuki
Affiliation:
Material Analysis Department, NISSAN ARC, LTD., 1 Natsushima-cho, Yokosuka, Kanagawa 237-0061, Japan
Takashi Harumoto
Affiliation:
Department of Materials Science and Technology, Faculty of Industrial Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-8510, Japan
Makoto Yuasa
Affiliation:
Department of Pure and Applied Chemistry, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-8510, Japan
Takashi Ishiguro
Affiliation:
Department of Materials Science and Technology, Faculty of Industrial Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-8510, Japan
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Abstract

Liposome was synthesized by using mixture of dipalmitoylphosphatidylcholine and cholesterol in the ultrapure water or physiological saline. Phase transformation temperature and vibrational mode of dipalmitoylphosphatidylcholine molecule were detected by using transmission Fourier-transform infrared spectroscopy for aqueous solution, which we developed. The liposomes were fixed on an amorphous carbon mesh for ultra-high resolution transmission electron microscopy observation and stained with platinum thymidine blue. As-prepared liposomes reinforced with cholesterol were spherical in shape with size larger than 100 nm in diameter and still stable in the vacuum. Under the strong electron irradiation condition, the solution enclosed in the liposomes became unstable and then collapsed. On the other hand, the liposome synthesized in the physiological saline sometimes contains crystallized salt. As a result, the liposome shows proper strength to hold wet material in itself in a vacuum and can be used for the transmission electron microscopy observation.

Keywords

biologicalinfrared (IR) spectroscopytransmission electron microscopy (TEM)
Type
Articles
Information
MRS Online Proceedings Library (OPL) , Volume 1498: Symposium R – Biomimetic, Bio-inspired and Self-Assembled Materials for Engineered Surfaces and Applications , 2013 , pp. 227 - 232
Copyright
Copyright © Materials Research Society 2013 

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References

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