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Synthesis of functionalized-thermo responsive-water soluble co-polymer for conjugation to protein for biomedical applications

Published online by Cambridge University Press:  25 January 2013

Ali Fathi ,
Hua Wei ,
Wojciech Chrzanowski ,
Anthony S. Weiss  and
Fariba Dehghani
Ali Fathi
Affiliation:
School of Chemical and Biomolecular Engineering, the University of Sydney, NSW 2006, Australia
Hua Wei
Affiliation:
School of Chemical and Biomolecular Engineering, the University of Sydney, NSW 2006, Australia
Wojciech Chrzanowski
Affiliation:
Faculty of Pharmacy, the University of Sydney, NSW 2006, Australia,
Anthony S. Weiss
Affiliation:
School of Molecular Bioscience, the University of Sydney, NSW 2006, Australia,
Fariba Dehghani
Affiliation:
School of Chemical and Biomolecular Engineering, the University of Sydney, NSW 2006, Australia
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Abstract

The aim of this study was to develop a thermo-responsive and bioactive polymer with suitable mechanical properties for musculoskeletal tissue engineering applications. A copolymer was synthesized that comprised of hydrophilic polyethylene glycol, thermo responsive N-isopropylacrylamide (NIPAAm), 2-hydroxyethyl methacrylate-poly(lactide) (HEMA-PLA) to enhance mechanical strength and an active N-acryloxysuccinimide (NAS) group for conjugation to proteins to enhance biological properties. A model protein such as elastin was used to assess the feasibility of conjugating this polymer to protein. The results of 1HNMR analyses confirmed that random polymerization was viable technique for synthesis of this copolymer. The co-polymers synthesized with PEG content of 3 mol% were water soluble. A hydrogel was created by dissolving the copolymer and elastin below room temperature in aqueous media, followed by rapid gelation at 37°C. The results of Fourier transform infrared analyses confirmed the conjugation of protein to copolymer due to significant reduction of ester group absorption (1735 cm−1). This data confirmed molecular interaction between protein and the temperature responsive co-polymer. Our preliminary results demonstrated that it is viable to tune different properties of this hydrogel by changing the composition of co-polymer.

Keywords

tissuepolymerizationbiomaterial
Type
Articles
Information
MRS Online Proceedings Library (OPL) , Volume 1498: Symposium L – Biomimetic, Bio-inspired and Self-Assembled Materials for Engineered Surfaces and Applications , 2013 , pp. 121 - 125
Copyright
Copyright © Materials Research Society 2013 

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