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Surface Modification for Protein Resistance Using a Biomimetic Approach

Published online by Cambridge University Press:  15 February 2011

Jeffrey L. Dalsin  and
Phillip B. Messersmith
Jeffrey L. Dalsin
Affiliation:
Biomedical Engineering Department andInstitute for Bioengineering and Nanoscience in Advanced Medicine(IBNAM), Northwestern University, Evanston, IL 60208
Phillip B. Messersmith
Affiliation:
Biomedical Engineering Department andInstitute for Bioengineering and Nanoscience in Advanced Medicine(IBNAM), Northwestern University, Evanston, IL 60208
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Abstract

In recent years the immobilization of poly(ethylene glycol) (PEG) on surfaces has proved to be one of the most attractive methods to prevent biological fouling of surfaces. We have developed a paradoxical biomimetic PEGylation strategy that exploits the adhesive characteristics of proteins secreted by marine mussels—one of nature's most notorious foulers. Linear PEGs were coupled to peptides containing 3,4-dihydroxyphenylalanine (DOPA), an unusual amino acid which is found in high concentration in these so-called mussel adhesive proteins. Using surface plasmon resonance, we have demonstrated enhanced resistance to protein adhesion on gold substrates modified with the DOPA-containing PEGs.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 774: Symposium O – Materials Inspired by Biology , 2003 , O2.2
Copyright
Copyright © Materials Research Society 2003

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