Hostname: page-component-cd9895bd7-q99xh Total loading time: 0 Render date: 2024-12-27T02:11:42.465Z Has data issue: false hasContentIssue false
  • English
  • Français

Surface Enhanced Vibrational Spectroscopy of Proteins with Plasmonic Nanoantenna Arrays

Published online by Cambridge University Press:  17 April 2019

Ronen Adato ,
Ahmet A. Yanik ,
Jason J. Amsden ,
David Kaplan ,
Fiorenzo Omenetto ,
Mi K. Hong ,
Shyamsunder Erramilli  and
Hatice Altug
Ronen Adato
Affiliation:
Department of Electrical and Computer Engineering, Boston University,8 St. Mary’s St., Boston, MA 02215, U.S.A Photonics Center, Boston University, 8 St. Mary’s St., Boston, MA, 02215
Ahmet A. Yanik
Affiliation:
Department of Electrical and Computer Engineering, Boston University,8 St. Mary’s St., Boston, MA 02215, U.S.A Photonics Center, Boston University, 8 St. Mary’s St., Boston, MA, 02215
Jason J. Amsden
Affiliation:
Department of Biomedical Engineering, Tufts University, 4 Colby St., Medford, MA, 02155
David Kaplan
Affiliation:
Department of Biomedical Engineering, Tufts University, 4 Colby St., Medford, MA, 02155
Fiorenzo Omenetto
Affiliation:
Department of Biomedical Engineering, Tufts University, 4 Colby St., Medford, MA, 02155 Department of Physics, Tufts University, 4 Colby St., Medford, MA, 02155
Mi K. Hong
Affiliation:
Department of Physics, Boston University, 590 Commonwealth Ave., Boston, MA, 02215 Photonics Center, Boston University, 8 St. Mary’s St., Boston, MA, 02215
Shyamsunder Erramilli
Affiliation:
Department of Physics, Boston University, 590 Commonwealth Ave., Boston, MA, 02215 Department of Biomedical Engineering, Boston University, 44 Cummington St., Boston, MA Photonics Center, Boston University, 8 St. Mary’s St., Boston, MA, 02215
Hatice Altug*
Affiliation:
Department of Electrical and Computer Engineering, Boston University,8 St. Mary’s St., Boston, MA 02215, U.S.A Materials Science Division, Boston University, 8 St. Mary’s St., Boston, MA, 0221 Photonics Center, Boston University, 8 St. Mary’s St., Boston, MA, 02215
*
*Corresponding author, [email protected]
Get access

Abstract

Infrared absorption spectroscopy is a powerful tool for structural and functional studies of biomolecules. The technique enables direct access to the vibrational fingerprints of molecular bonds in the mid-infrared spectral region (3-20μm). Although intrinsic absorption cross-sections are nearly ten orders of magnitude greater than corresponding Raman cross-sections, they are still small in comparison with those of fluorescent molecules. Sensitivity improvements are therefore required for the method to be applicable to single molecule / molecular layer studies. In this work, we demonstrate the use of lithographically patterned arrays of nanoantennas to enhance the absorption signature of the protein amide-I and II backbone vibrations. Strong absorption signals from monolayer thickness films are obtained. By arranging ensembles of tailored antennas in specific lattices, higher quality factor resonances and increased near-field intensities are possible. These features are leveraged to obtain 104-105 fold signal enhancements and the direct measurement of vibrational spectra of proteins at zepto-mole sensitivity levels.

Keywords

biomedicalinfrared (IR) spectroscopynanoscale
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1248: Symposium D – Solution Processing of Inorganic and Hybrid Materials for Electronics and Photonics , 2010 , 1248-D10-02
Copyright
Copyright © Materials Research Society 2010

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

1. Lal, S., Link, S. and Halas, N. J., Nature Photon. 1, 641 (2007).Google Scholar
2. Kneipp, K., Wang, Y., Kneipp, H., Perelman, L. T., Itzkan, I., Dasari, R. R. and Feld, M. S., Phys. Rev. Lett. 78, 1667 (1997).Google Scholar
3. Osawa, M. and Ikeda, M., J. Phys. Chem. 95, 9914 (1991).Google Scholar
4. Neubrechh, F., Pucci, A., Cornelius, T. W., Karim, S., Garcia-Extarri, A. and Aizpurua, J., Phys. Rev. Lett. 101, 157403 (2008)Google Scholar
5. Adato, R., Yanik, A. A., Amsden, J. J., Kaplan, D., Omenetto, F., Hong, M. K., Erramilli, S. and Altug, H., Proc. Nat. Acad. Sci. USA, 106, 19227 (2009).Google Scholar
6. Adato, R., Yanik, A. A., Wu, C.-H., Shvets, G. and Altug, H., Opt. Express 18, 4526 (2010).Google Scholar
7. Zou, S., Janel, N. and Schatz, G. C., J. Chem. Phys. 120, 10871 (2004).Google Scholar
8. Lawrence, B. D., Omenetto, F. G., Chi, K. and Kaplan, D. L., J. Mater. Sci. 43, 6967 (2008).Google Scholar
9. Novotny, L., Phys. Rev. Lett., 98, 266802 (2007).Google Scholar