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Surface Enhanced Vibrational Spectroscopy of Proteins with Plasmonic Nanoantenna Arrays

Published online by Cambridge University Press:  17 April 2019

Ronen Adato ,
Ahmet A. Yanik ,
Jason J. Amsden ,
David Kaplan ,
Fiorenzo Omenetto ,
Mi K. Hong ,
Shyamsunder Erramilli  and
Hatice Altug
Ronen Adato
Affiliation:
Department of Electrical and Computer Engineering, Boston University,8 St. Mary’s St., Boston, MA 02215, U.S.A Photonics Center, Boston University, 8 St. Mary’s St., Boston, MA, 02215
Ahmet A. Yanik
Affiliation:
Department of Electrical and Computer Engineering, Boston University,8 St. Mary’s St., Boston, MA 02215, U.S.A Photonics Center, Boston University, 8 St. Mary’s St., Boston, MA, 02215
Jason J. Amsden
Affiliation:
Department of Biomedical Engineering, Tufts University, 4 Colby St., Medford, MA, 02155
David Kaplan
Affiliation:
Department of Biomedical Engineering, Tufts University, 4 Colby St., Medford, MA, 02155
Fiorenzo Omenetto
Affiliation:
Department of Biomedical Engineering, Tufts University, 4 Colby St., Medford, MA, 02155 Department of Physics, Tufts University, 4 Colby St., Medford, MA, 02155
Mi K. Hong
Affiliation:
Department of Physics, Boston University, 590 Commonwealth Ave., Boston, MA, 02215 Photonics Center, Boston University, 8 St. Mary’s St., Boston, MA, 02215
Shyamsunder Erramilli
Affiliation:
Department of Physics, Boston University, 590 Commonwealth Ave., Boston, MA, 02215 Department of Biomedical Engineering, Boston University, 44 Cummington St., Boston, MA Photonics Center, Boston University, 8 St. Mary’s St., Boston, MA, 02215
Hatice Altug*
Affiliation:
Department of Electrical and Computer Engineering, Boston University,8 St. Mary’s St., Boston, MA 02215, U.S.A Materials Science Division, Boston University, 8 St. Mary’s St., Boston, MA, 0221 Photonics Center, Boston University, 8 St. Mary’s St., Boston, MA, 02215
*
*Corresponding author, [email protected]
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Abstract

Infrared absorption spectroscopy is a powerful tool for structural and functional studies of biomolecules. The technique enables direct access to the vibrational fingerprints of molecular bonds in the mid-infrared spectral region (3-20μm). Although intrinsic absorption cross-sections are nearly ten orders of magnitude greater than corresponding Raman cross-sections, they are still small in comparison with those of fluorescent molecules. Sensitivity improvements are therefore required for the method to be applicable to single molecule / molecular layer studies. In this work, we demonstrate the use of lithographically patterned arrays of nanoantennas to enhance the absorption signature of the protein amide-I and II backbone vibrations. Strong absorption signals from monolayer thickness films are obtained. By arranging ensembles of tailored antennas in specific lattices, higher quality factor resonances and increased near-field intensities are possible. These features are leveraged to obtain 104-105 fold signal enhancements and the direct measurement of vibrational spectra of proteins at zepto-mole sensitivity levels.

Keywords

biomedicalinfrared (IR) spectroscopynanoscale
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1248: Symposium D – Solution Processing of Inorganic and Hybrid Materials for Electronics and Photonics , 2010 , 1248-D10-02
Copyright
Copyright © Materials Research Society 2010

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