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Sporopollenin microcapsules for microencapsulation of living cells

Published online by Cambridge University Press:  10 April 2013

Shwan A. Hamad ,
Amro K.F. Dyab ,
Simeon D. Stoyanov  and
Vesselin N. Paunov
Shwan A. Hamad
Affiliation:
Surfactant & Colloid Group, Department of Chemistry, University of Hull, Hull, HU6 7RX, UK.
Amro K.F. Dyab
Affiliation:
Surfactant & Colloid Group, Department of Chemistry, University of Hull, Hull, HU6 7RX, UK. Surfactant Research Chair, Chemistry Department, College of Science, King Saud University, P.O.Box - 2455, Riyadh - 11451, Kingdom of Saudi Arabia.
Simeon D. Stoyanov
Affiliation:
Unilever Foods and Health Research Institute, Olivier van Noortlaan 120, 3133 AT Vlaardingen, The Netherlands.
Vesselin N. Paunov*
Affiliation:
Surfactant & Colloid Group, Department of Chemistry, University of Hull, Hull, HU6 7RX, UK.
*
*Email: [email protected], Phone : +44 (0) 1482 465660, Fax : +44 (0) 1482 466410.
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Abstract

We used sporopollenin prepared from Lycopodium Clavatum to encapsulate living yeast cells as a model for probiotics. The microencapsulation of cells was achieved by using the trilite scars of the sporopollenin microcapsules which can open up by compressing the sporopollenin into a pellet. Such compressed pellets were exposed to an aqueous suspension of yeast cells in the presence of a biocompatible surface active agent which allowed living cells to be loaded inside the sporopollenin particles by the influx of liquid to the sporopollenin interior as the deformed microcapsules re-inflated to their original state. We demonstrated that the cells viability and biological activity is preserved after the microencapsulation in the sporopollenin. Such microencapsulation technology could find application in preserving cells from mechanical stress and aggressive environments which can be used in protection of probiotics in food formulations.

Keywords

colloidscanning electron microscopy (SEM)biomaterial
Type
Articles
Information
MRS Online Proceedings Library (OPL) , Volume 1499: Symposium N – Precision Polymer Materials―Fabricating Functional Assemblies, Surfaces, Interfaces and Devices , 2013 , mrsf12-1499-n05-181
Copyright
Copyright © Materials Research Society 2013

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References

REFERENCES

Zetzsche, F., Kälin, O., Helvetica Chimica Acta, 14, 517 (1931).CrossRefGoogle Scholar
Faegri, I., I., Iverson, J. Textbook of Pollen Analysis: Oxford, 1984.Google Scholar
(a) Brooks, J., Shaw, G., Origin and Development of Living Systems Academic Press: London and New York, 1973; (b) J. Brooks, G., Shaw, Nature, 219, 532 (1968); (c) G. Shaw, Phytochemical Phylogeny Academic Press: London and New York, 1970; (d) J. Brooks, M.D. Muir, G. Shaw, Nature, 244, 215 (1973); (e) G. Shaw, Pollen: Development and Physiology; Butherworths: London, 1971.Google Scholar
Wittborn, J., Rao, K.V., El-Ghazaly, G., Rowley, J.R., Annals of Botany, 82, 141 (1998).CrossRefGoogle Scholar
Bohne, G., Richter, E., Woehlecke, H., Ehwaldi, R., Annals of Botany, 92, 289 (2003).CrossRefGoogle Scholar
Straka, H., Pollen und Sporenkunde; Gustav Fischer Verlag: Stuttgart, 1975 Google Scholar
(a) Blackwell, L., PhD Thesis, University of Hull, 2008; (b) M. Lorch, M.J. Thomasson, A. Diego-Taboada, S. Barrier, S.L. Atkin, G. Mackenzie, S. Archibald, J. Chem. Commun., 6442 (2009).Google Scholar
(a) Barrier, S., Diego-Taboada, A., Thomasson, M.J., Madden, L., Pointon, J.C., Wadhawan, J.D., Beckett, S.T., Atkin, S.L., Mackenzie, G., J. Mater. Chem., 21, 975 (2011); (b) S. Barrier, A.S. Rigby, A. Diego-Taboada, M.J. Thomasson, G., Mackenzie, S.L. Atkin, Lwt-Food Science and Technology, 43, 73 (2010); (c) S.T. Beckett, G. Mackenzie, Chem. Rev., 20, 10 (2010); (d) A. Wakil, G. Mackenzie, A. Diego-Taboada, J.G. Bell, S.L. Atkin, Lipids, 45, 645 (2010); (e) G. Mackenzie, S.L. Atkin, A. Digeo-Taboada, S. Barrier, M.J. Thomasson, Innov. in Pharm. Tech., 63. (2007).CrossRefGoogle Scholar
Paunov, V.N., Mackenzie, G., Stoyanov, S.D., J. Mater. Chem., 17, 609 (2007).CrossRefGoogle Scholar
Hamad, S.A., Dyab, A.K.F., Stoyanov, S.D., Paunov, V.N., J. Mater. Chem., 21, 18018 (2011).CrossRefGoogle Scholar
Hamad, S.A., Stoyanov, S.D., Paunov, V.N., Soft Matter, 8, 5069 (2012).CrossRefGoogle Scholar