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Short-term Cytotoxic and Inflammatory Responses of Human Monocytes to Stainless Steel Fibre Networks

Published online by Cambridge University Press:  23 April 2012

Rose L. Spear ,
Roger A. Brooks  and
Athina E. Markaki
Rose L. Spear
Affiliation:
Department of Engineering, University of Cambridge, Trumpington Street, Cambridge, CB2 1PZ, UK
Roger A. Brooks
Affiliation:
Orthopaedic Research Unit, Addenbrooke’s Hospital, Hills Road, Cambridge, CB2 2QQ, UK
Athina E. Markaki
Affiliation:
Department of Engineering, University of Cambridge, Trumpington Street, Cambridge, CB2 1PZ, UK
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Abstract

The aim of the current work was to examine the human monocyte response to 444 ferritic stainless steel fibre networks. 316L austenitic fibre networks, of the same fibre volume fraction, were used as control surfaces. Fluorescence and scanning electron microscopies suggest that the cells exhibited a good degree of attachment and penetration throughout both networks. Lactate Dehydrogenase (LDH) and TNF-α releases were used as indicators of cytotoxicity and inflammatory responses respectively. LDH release indicated similar levels of monocyte viability when in contact with the 444 and 316L fibre networks. Both networks elicited a low level secretion of TNF-α, which was significantly lower than that of the positive control wells containing zymosan. Collectively, the results suggest that 444 ferritic and 316L austenitic networks induced similar cytotoxic and inflammatory responses from human monocytes.

Keywords

steelbiomedicalbiological
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1417: Symposium KK – Biomaterials for Tissue Regeneration , 2012 , mrsf11-1417-kk06-23
Copyright
Copyright © Materials Research Society 2012

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References

REFERENCES

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