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Production of Highly Ordered Nanoporous Alumina and its Application in Cell Cultivation

Published online by Cambridge University Press:  01 February 2011

Andreas Hoess ,
Andrea Staeudte ,
Annika Thormann ,
Martin Steinhart  and
Andreas Heilmann
Andreas Hoess
Affiliation:
[email protected], Fraunhofer Institute for Mechanics of Materials, Biological Materials and Interfaces, Walter-Hülse-Strasse 1, Halle, 06120, Germany, +49345 5589286, +49345 5589101
Andrea Staeudte
Affiliation:
[email protected], Fraunhofer Institute for Mechanics of Materials, Biological Materials and Interfaces, Walter-Hülse-Strasse 1, Halle, 06120, Germany
Annika Thormann
Affiliation:
[email protected], Fraunhofer Institute for Mechanics of Materials, Biological Materials and Interfaces, Walter-Hülse-Strasse 1, Halle, 06120, Germany
Martin Steinhart
Affiliation:
[email protected], Max Planck Institute of Microstructure Physics, Experimental Department II, Weinberg 2, Halle, 06120, Germany
Andreas Heilmann
Affiliation:
[email protected], Fraunhofer Institute for Mechanics of Materials, Biological Materials and Interfaces, Walter-Hülse-Strasse 1, Halle, 06120, Germany
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Abstract

Nanoporous alumina membranes were utilized as cell culture substrates. Two different anodization processes were applied in order to obtain highly ordered, self-supporting nanoporous alumina membranes. The resulting membranes exhibit uniform nanopores with constant diameters having aspect ratios (pore depth:pore diameter) larger than 1000. Tentative experiments evaluating the cellular interaction of a hepatoma cell line (HepG2) with the nanoporous alumina membranes will be described. Furthermore, cell proliferation as well as the adhesion behavior of the cells on the porous substrates was investigated. Scanning electron microscopy (SEM) and focused ion beam (FIB) technology were applied to examine the cell morphology. The experiments revealed that the nanoporous membranes can be used as cell culture substrates and that they have no adverse effects on cell proliferation and cell viability. Besides, cells on membranes with pore diameters larger than 200 nm developed small cell extensions (filopodia) which penetrated into the nanopores.

Keywords

nanostructurebiomaterialporosity
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1093: Symposium CC – Designer Biointerfaces , 2008 , 1093-CC04-16
Copyright
Copyright © Materials Research Society 2008

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