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Probing the Viscoelasticity of Collagen Solutions via Optical-Tweezers-Based Microrheology

Published online by Cambridge University Press:  23 May 2012

Marjan Shayegan  and
Nancy R. Forde
Marjan Shayegan
Affiliation:
Simon Fraser University, Department of Chemistry, 8888 University Dr., Burnaby, BC, V5A 1S6, Canada
Nancy R. Forde
Affiliation:
Simon Fraser University, Department of Physics, 8888 University Dr., Burnaby, BC, V5A 1S6, Canada
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Abstract

How the molecular structure of proteins in solution correlates with the mechanical properties of the solution at different length scales is not known. Using optical-tweezers based microrheology, we investigate a key physical property, viscoelasticity, of collagen solutions. To do this, we measure short-range thermal fluctuations of probe particles to obtain elastic and viscous moduli of their surrounding medium, and validate our measurement and analysis techniques using the previously studied system of polyethylene oxide. Probing the concentration dependence of viscoelasticity, we find that collagen solutions exhibit elasticity of comparable strength to viscosity when the concentration reaches ∼5 mg/ml. We also find that the presence of telopeptides alters the viscoelasticity of collagen solutions, particularly at high frequencies

Keywords

viscoelasticitybiomaterialmicrostructure
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1465: Symposium SS – Structure/Property Relationships in Biological and Biomimetic Materials at the Micro-, Nano- and Atomic-Length Scales , 2012 , mrss12-1465-ss06-08
Copyright
Copyright © Materials Research Society 2012

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References

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