Hostname: page-component-586b7cd67f-vdxz6 Total loading time: 0 Render date: 2024-11-26T13:09:27.949Z Has data issue: false hasContentIssue false
  • English
  • Français

A Portable Setup for Molecular Detection by Transmission LSPR

Published online by Cambridge University Press:  04 February 2013

Giulia Cappi ,
Enrico Accastelli ,
Fabio M. Spiga ,
Vera Cantale ,
Maria A. Rampi ,
Luca Benini  and
Carlotta Guiducci
Giulia Cappi*
Affiliation:
Laboratory of Life Sciences Electronics, Swiss Federal Institute of Technology (EPFL), 1015 Lausanne, Switzerland.
Enrico Accastelli
Affiliation:
Laboratory of Life Sciences Electronics, Swiss Federal Institute of Technology (EPFL), 1015 Lausanne, Switzerland.
Fabio M. Spiga
Affiliation:
Laboratory of Life Sciences Electronics, Swiss Federal Institute of Technology (EPFL), 1015 Lausanne, Switzerland.
Vera Cantale
Affiliation:
Dipartimento di Chimica, Università di Ferrara, via Borsari 46, 44121 Ferrara, Italy.
Maria A. Rampi
Affiliation:
Dipartimento di Chimica, Università di Ferrara, via Borsari 46, 44121 Ferrara, Italy.
Luca Benini
Affiliation:
Dipartimento di Elettronica, Informatica e Sistemistica, Università di Bologna, Viale Risorgimento 2, 40136 Bologna, Italy.
Carlotta Guiducci
Affiliation:
Laboratory of Life Sciences Electronics, Swiss Federal Institute of Technology (EPFL), 1015 Lausanne, Switzerland.
*
*Contact author’s e-mail: [email protected]
Get access

Abstract

In the framework of bioanalytics and multiple array detection, we developed a fully portable and low-cost detection system based on Localized Surface Plasmon Resonance (LSPR) in a transmission configuration (T-LSPR). The transmission approach is suitable to be scaled to small dimension systems and to enable high-density array measurements on the same platform. Our setup is made out of off-the-shelf components and consists of a set of discrete light sources and a couple of light-detectors which enable a differential measurement setup. An algorithm fits the measured data and extracts the information of the plasmon peak position in the spectrum.

The performance of our T-LSPR measurement system has been characterized on a set of Fluorinated Tin Oxide-coated glass slides covered with gold Nanoislands (NIs). The samples have been modified with a single-stranded DNA layer and a real-time DNA hybridization experiment has been performed. Here we demonstrate that the proposed T-LSPR device, based on the characterization of the plasmon peak with a differential approach, is able to monitor real-time DNA hybridization on surface, and to precisely measure the position of the peak with a standard deviation in wavelength of 0.2 nm.

Keywords

sensorbiologicaloptical
Type
Articles
Information
MRS Online Proceedings Library (OPL) , Volume 1479: Symposium S – Nanostructured Materials and Nanotechnology—2012 , 2012 , pp. 27 - 32
Copyright
Copyright © Materials Research Society 2012

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Svedendahl, M, Chen, S, Dmitriev, A, Käll, M. Nano Letters 9: 4428–33 (2009).CrossRefGoogle Scholar
Doron-Mor, I, Cohen, H, Barkay, Z, Shanzer, A, Vaskevich, A, Rubinstein, I. Chemistry – A European Journal 11: 5555–62 (2005).CrossRefGoogle Scholar
Ruach-Nir, I, Bendikov, TA, Doron-Mor, I, Barkay, Z, Vaskevich, A, Rubinstein, I. J Am Chem Soc 129: 84–92 (2006).CrossRefGoogle Scholar
Hutter, E, Pileni, M-P. The Journal of Physical Chemistry B 107: 6497–9 (2003).CrossRefGoogle Scholar
Anker, JN, Hall, WP, Lyandres, O, Shah, NC, Zhao, J, Van Duyne, RP. Nat Mater 7: 442–53 (2008).CrossRefGoogle Scholar
Miller, MM, Lazarides, AA. The Journal of Physical Chemistry B 109: 21556–65 (2005).CrossRefGoogle Scholar
Malinsky, MD, Kelly, KL, Schatz, GC, Van Duyne, RP. J Am Chem Soc 123: 1471–82 (2001).CrossRefGoogle Scholar
Haynes, CL, Van Duyne, RP. The Journal of Physical Chemistry B 105: 5599–611 (2001).CrossRefGoogle Scholar
Willets, KA, Van Duyne, RP. Annu Rev Phys Chem 58: 267–97 (2007).CrossRefGoogle Scholar
Cantale, V, Simeone, FC, Gambari, R, Rampi, MA. Sensors and Actuators B: Chemical 152: 206–13 (2011).CrossRefGoogle Scholar
Montmeat, P, Marchand, J-C, Lalauze, R, Viricelle, J-P, Tournier, G, Pijolat, C. Sensors and Actuators B: Chemical 95: 83–9 (2003).CrossRefGoogle Scholar
Cappi, G, Accastelli, E, Cantale, V, Rampi, MA, Benini, L, Guiducci, C. Sens. Actuators, B In press.Google Scholar