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Polymeric Tissue Culture Substrates patterned by UV Irradiation

Published online by Cambridge University Press:  17 March 2011

Alexander Welle ,
Eric Gottwald ,
Karl-Friedrich Weibezahn  and
Herman Dertinger
Alexander Welle
Affiliation:
Forschungszentrum Karlsruhe GmbH, Institute for Medical Engineering and BiophysicsP.O. Box 3640, 76021 Karlsruhe, GERMANY
Eric Gottwald
Affiliation:
Forschungszentrum Karlsruhe GmbH, Institute for Medical Engineering and BiophysicsP.O. Box 3640, 76021 Karlsruhe, GERMANY
Karl-Friedrich Weibezahn
Affiliation:
Forschungszentrum Karlsruhe GmbH, Institute for Medical Engineering and BiophysicsP.O. Box 3640, 76021 Karlsruhe, GERMANY
Herman Dertinger
Affiliation:
Forschungszentrum Karlsruhe GmbH, Institute for Medical Engineering and BiophysicsP.O. Box 3640, 76021 Karlsruhe, GERMANY
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Abstract

We studied the physico/chemical effects of deep UV irradiation of polystyrene, PMMA and polycarbonate with respect to cell adhesion and protein immobilization. Photochemical modifications of the polymer surfaces yielded unstable peroxides and carboxylic acid groups. Patterned protein adsorbates were realized by coupling via carbodiimid activation of the COOH moieties. We have immobilized enzymes and antibodies. It was observed that hepatoma cells (HepG2) and fibroblasts (L929) adhered in the presence of serum proteins in the culture medium on the irradiated regions of the substrate without any further treatment.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 711: Symposia FF/GG/HH – Advanced Biomaterials--Characterization, Tissue Engineering and Complexity , 2001 , GG1.6.1
Copyright
Copyright © Materials Research Society 2002

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References

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