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Polyethylene Glycol Deposition Techniques for Antifouling Surfaces: Using Antistiction to Conserve Bioparticles for Recovery and Analysis

Published online by Cambridge University Press:  01 February 2011

Norine E. Chang ,
Meng H. Lean  and
Scott J. Limb
Norine E. Chang
Affiliation:
[email protected], Palo Alto Research Center, Hardware Systems Laboratory, 3333 Coyote Hill Rd, Palo Alto, CA, 94304, United States
Meng H. Lean
Affiliation:
[email protected], Palo Alto Research Center, Hardware Systems Laboratory, 3333 Coyote Hill Rd., Palo Alto, CA, 94304, United States
Scott J. Limb
Affiliation:
[email protected], Palo Alto Research Center, Hardware Systems Laboratory, 3333 Coyote Hill Rd., Palo Alto, CA, 94304, United States
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Abstract

For bioparticle analysis within microfluidic devices, there is a risk of analytes adhering to surfaces, thereby compromising particle manipulation and recovery. To address this we have implemented polyethylene glycol (PEG)-type coatings by self-assembled monolayer (SAM) and plasma-polymerizing deposition techniques. Silane chemistry is used to deposit SAM films directly onto silicon dioxide surfaces, and in a special case for which a composite of metallic arrays and SiON within our MEMS device must be coated, an ultrathin layer of sputtered Si is added before the SAM protocol. We have also deposited PEG-like tetraglyme by plasma-polymerization onto substrates using an onsite-built reactor to provide antistiction treatment to all surfaces, including those that do not have chemical compatibility for the SAM technique. Three tests demonstrate the effectiveness of our surface treatments: static exposure to microbial suspensions, bioparticle transport across MEMS traveling-wave (TW) arrays, and bioparticle recovery from a circulating flow device. Our static microbial assays show significant reduction in B. thuringiensis adhesion to both the SAM and plasma-polymerized coatings and reduction in B. globigii adhesion to the plasma-polymerized coating. Our TW arrays, when coated with either the SAM or plasma-polymerized PEG film, are effective at reducing adhesion to polystyrene beads as well as both Bacillus species. Lastly, our bioparticle recovery, as gauged by spectrophotometry, improves by as much as one order of magnitude when we coat flow chambers with our plasma-polymerized film.

Keywords

biologicalsurface chemistryadhesion
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 954: Symposium H – Biofilm-Material Interactions — New Tools, Technologies and Opportunities , 2006 , 0954-H03-03
Copyright
Copyright © Materials Research Society 2007

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References

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