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Plasma Protein Adsorption and Platelet Adhesion on Poly[Bis(Trifluoroethoxy)Phosphazene]

Published online by Cambridge University Press:  15 February 2011

Alexander Welle ,
Michael Grunze  and
Dsidra Tur
Alexander Welle
Affiliation:
University Heidelberg, Institute of Applied Physical Chemistry, Germany
Michael Grunze
Affiliation:
University Heidelberg, Institute of Applied Physical Chemistry, Germany
Dsidra Tur
Affiliation:
Academy of Sciences, Institute of Organo-Element Compounds, Moscow, Russia
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Abstract

Poly[bis(trifluoroethoxy)phosphazene] (PTFEP) with a high purity and high molar mass is a biocompatible material [1] used as bulk material in medical implants. We developed a process to coat surfaces with PTFEP films and performed ELISA experiments designed to understand their blood compatibility. We observed that PTFEP adsorbs preferentially albumin from plasma, and only small amounts of coagulation or inflammation stimulating proteins. In general, there is a good correlation between increasing content of albumin in the adsorbed protein film and reduced platelet adhesion. Another important prerequisite of blood compatibility is the stabilization of the native state of adsorbed proteins, since denaturated proteins stimulate platelet adhesion. The elutability of adsorbed proteins by sodiumdodecylsulfate solution was used to quantify the amount of irreversible attached and presumably denaturated proteins. PTFEP showed a low amount of irreversibly adsorbed proteins of the coagulation cascade. Circular dichroism measurements of adsorbed fibrinogen and albumin showed only weak distortions of the secondary structure of these proteins on the surface of PTFEP.

We conclude that PTFEP has a unique blood compatibility because of the favorable composition and the stabilization of the protein layer against denaturation.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 489: Symposium K – Materials Science of the Cell , 1997 , 139
Copyright
Copyright © Materials Research Society 1998

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References

[1] Tur, D.S., Acta Polymerica; 36, p. 627, (1985)Google Scholar
[2] Tamada, Y., Ikada, Y, J. Colloid and Interface Sci., 155, p. 334, (1993)Google Scholar
[3] Hechner, J.F., Edwards, R.O., J. Biomed. Mat. Res.; 15, p. 1, (1981)Google Scholar
[4] Sawyer, P.N., Srinivasan, S., Am. J. Surg., 114, p. 42, (1967)Google Scholar
[5] Blomberg, E., Claesson, P.M., Tilton, R.D., J. Colloid Interf. Sci., 166, p. 427, (1994)Google Scholar
[6] Lenk, T.J., Horbett, T.A., Ratner, B.D., Langmuir, 7, p. 1755, (1991)Google Scholar
[7] Allcock, H.R., Angewandte Chemie, 89, p. 153, (1977)Google Scholar
[8] Lora, S., Carenza, M., Palma, G., Pezzin, G., Caliceti, P., Battaglia, P., Lora, A., Biomaterials, 12, p. 275, (1991)Google Scholar
[9] Stenger, D.A., Georger, J.H., Dulcey, C.S., Hickman, J.J., Rudolph, A.S., Nielsen, T.B., McCort, S.M., Calvert, J.M., J. Am. Chem. Soc., 114, p. 8435, (1992)Google Scholar
[10] Tur, D.S. Acta Polymerica, 33, p. 331, (1985) Tur, D.S., Acta Polymerica, 39, p. 424, (1988)Google Scholar
[11] Renken, J., Dahint, J., Grunze, M., Josse, F., Anal. Chem., 68, p. 176, (1996)Google Scholar
[12] Dekker, A., Reitsma, K., Beugeling, T., Bantjes, A., Feijen, J., van Aken, W.G., Biomaterials, 12, p. 130, (1991)Google Scholar
[13] Tang, L., Eaton, J.W., J. Exp. Med., 178, p. 2147, (1993)Google Scholar
[14] Breddin, K., Grun, H., Krzywanek, J., Schremmer, W.P., Klin. Wschr., 53, p. 81, (1975)Google Scholar