Hostname: page-component-586b7cd67f-dsjbd Total loading time: 0 Render date: 2024-11-23T18:02:01.562Z Has data issue: false hasContentIssue false
  • English
  • Français

Piezoelectric Poly(3-hydroxybutyrate)-Poly(lactic acid) Three Dimensional Scaffolds for Bone Tissue Engineering

Published online by Cambridge University Press:  01 February 2011

Juana Mendenhall ,
Dapeng Li ,
Margaret Frey ,
Juan Hinestroza ,
Omotunde Babalola ,
Lawrence Bonnasar  and
Carl A Batt
Juana Mendenhall
Affiliation:
[email protected], GA Tech, Biomedical Engineering, 313 Ferst Drive Room 1221, Atlanta, GA, 30332, United States, 404-668-3197
Dapeng Li
Affiliation:
[email protected], Cornell University, Fiber Science and Apparel Design, Ithaca, NY, 14853, United States
Margaret Frey
Affiliation:
[email protected], Cornell University, Fiber Science and Apparel Design, Ithaca, NY, 14853, United States
Juan Hinestroza
Affiliation:
[email protected], Cornell University, Fiber Science and Apparel Design, Ithaca, NY, 14853, United States
Omotunde Babalola
Affiliation:
[email protected], Cornell University, Biomedical Engineering, Ithaca, NY, 14853, United States
Lawrence Bonnasar
Affiliation:
[email protected], Cornell University, Biomedical Engineering, Ithaca, NY, 14853, United States
Carl A Batt
Affiliation:
[email protected], Cornell University, Food Science, Ithaca, NY, 14853, United States
Get access

Abstract

Three dimensional scaffolds (3D) are promising for future nanoscale materials and tissue engineering applications being that they have architecture and mechanical properties similar to natural tissue. In this work, poly(lactic acid) fibers were prepared via electrospinnig with average diameters of 2580 nm. Using Enzymatic Surface-Initiated polymerization (ESIP), poly(3-hydroxybutyrate) were coated on poly(lactic acid) fibers. This provides an alternative method to enzymatic surface modification of fibers. ESIP of PHB produces a granular film providing surface topography and increases mechanical properties of PLA fibers alone. When using covalent approaches, PHB granules provide surface topography of 200-500 nm with a polydisperse coverage area. Compressive modulus measurements of PLA and PHB/PLA scaffolds were 25 kPa and 73 kPa, respectively. The percent crystallinity of PLA and PHB/PLA scaffolds was 17% and 30%, respectively. This rough topography, in addition to the crystallinity of the scaffold, facilitates Soas-2 osteoblast cell attachment. We have observed attachment of the osteoblast cells along the length of the oriented PLA and PHB/PLA composite scaffolds with different morphologies, rounded and stretched, throughout a depth of 90 µm.

Keywords

biomaterialfibertissue
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1025: Symposium B – Nanoscale Phenomena in Functional Materials by Scanning Probe Microscopy , 2007 , 1025-B12-03
Copyright
Copyright © Materials Research Society 2008

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

1. Doshi, J., Reneker, D.H. Electrospinning process and applications of Electrospun Fibers. J.Electrostat. 1995, 35, 2/3, 151–160.Google Scholar
2. Chen, G.Q. and Wu, Q. The Application of Polyhydroxyalkanoates as tissue engineering materials. Biomaterials. 2005, 26, 6565–6578.Google Scholar
3. Williams, SF., Martin, DP., Horowitz, DM., Peoples OP. PHA applications: addressing the price of performance issue I. Tissue Engineering. Int. J. Bio. Macro. 1999, 25, 111–121.Google Scholar
4. Freir, T., Kunze, C., Nischan, C., Kramer, S., Sternberg, K., Sass, M., Hopt, UT., Schmitz, KP. In vitro and in vivo degradation studies for development of biodegradable patch based on poly(3-hydroxybutyrate). Biomaterials. 2002, 23(13), 2649–2657.Google Scholar
5. Zhao, K., Deng, Y., Chen, C.J., Chen, G.Q. Polyhydroxyalkanoates (PHA) scaffolds with good mechanical properties and biocompatibility. Biomaterials. 2003, 24, 1041–1045.Google Scholar
6. Pruker, O., Rueche, J. Polymer Layers through Self-Assembled Monolayers of Initiators. Langmuir. 1998, 14, 6893–6898.Google Scholar
7. Jordan, R., Ulman, A., Kang, J.F., Rafailovich, M.H., Sokolov, J. Surface-Initiated Anionic Polymerization of Styrenes by means of Self-Assembled Monolayers. JACS. 1999, 121, 1016–1022.Google Scholar
8. Reith, L.R., Moore, D.R., Lobkovsky, E.B., Coates, G.W. Single-site-B-Diiminate Zince catalysts for the Ring-opening Polymerization of B-Butyrolactone and B-Valerolactone to Poly(3-hydroxyalkanoates. J.Am. Chem. Soc. 2002, 124, 15239–15248.Google Scholar
9. Matyjaszewski, K., Miller, P.J., Shukla, N., Immaraporn, B., Gelman, A., Luokala, B.B., Siclovan, T.M., Kickelbick, G., Vallant, T., Hoffman, H., Pakula, T. Polymers at Interfaces: Using Atomic Transfer Radical Polymerization in the controlled growth of homopolymers and Block copolymers from Silicon Surfaces in the absence of Untethred Sacrificial Initiators. Macromolecules. 1999, 32, 8716–8724.Google Scholar
10. Kim, Y-R., Paik, H.-j., Ober, C.K., Coates, G.W., Batt, C.A. Enzymatic Surface-Initiated Polymerization: A Novel Approach for the In-Situ Solid-Phase Synthesis of Biocompatible Polymer Poly(3-hydroxybutyrate). Biomacromolecules. 2004, 5, 889–894.Google Scholar
11. Li, D., Frey, M., Joo, Y. Characterization of nanofibrous membranes with capillary flow porometry. Journal of Membrane Sci. 2006, 286, 104–114.Google Scholar
12. Kim, Y.R., Nanoengineering of Solid Surfaces Using an In Vitro Synthesized Biological Polymer, Cornell University, Ithaca. 2003.Google Scholar
13. Yan, L., Marzolin, C., Terfort, A, Whitesides, G.M. Formation and Reaction of Interchain Carboxylic Anhydrides Groups on Self-Assembled Monolayers of Gold. Langmuir. 1997, 13, 6704.Google Scholar
14. Koyama, N. and Doi, Y. Miscibility of binary blends of poly[(R )-3-hydroxybutryric acid] and poly[(S)-lactic acid. Polymer. 1997, 38(7), 1589–1593.Google Scholar
15. Niamsiri, N., Bergkvist, M., Delamarre, S., Cady, N., Coates, G.W., Ober, C.K., Batt, C.A. Insight in the role of bovine serum albumin for promoting the in situ surface growth of polyhydroxybutyrate (PHB) on patterned surfaces via enzymatic surface-intitated polymerization. Colliods and Surfaces B: Biointerfaces. 2007, 60, 68–70 Google Scholar
16. Ouchi, T., Miyazaki, H., Arimura, H., Tasaka, F., Hamada, A., Ohya, Y., J. Polym. Sci., Part A: Polym. Chem. 2002, 40, 1218.Google Scholar
17. Wang, Y.W., Wu, Q., Chen, J., Chen, G.Q. Evaluation of three dimensional scaffolds made of blends of hydroxyapaptite and poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) for bone reconstruction. Biomaterials. 2005, 26, 899–904.Google Scholar